Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01260 and SGL_RS01265 overlap by 4 nucleotides SGL_RS01265 and SGL_RS01270 are separated by 217 nucleotides SGL_RS01270 and SGL_RS01275 are separated by 285 nucleotides
SGL_RS01260: SGL_RS01260 - putative toxin-antitoxin system toxin component, PIN family, at 23,434 to 23,859
_RS01260
SGL_RS01265: SGL_RS01265 - AbrB/MazE/SpoVT family DNA-binding domain-containing protein, at 23,856 to 24,077
_RS01265
SGL_RS01270: SGL_RS01270 - recombinase family protein, at 24,295 to 24,861
_RS01270
SGL_RS01275: SGL_RS01275 - DUF839 domain-containing protein, at 25,147 to 27,345
_RS01275
Position (kb) 24
25 Strain fitness (log2 ratio) -1
0
1
2
3 at 23.774 kb on - strand, within SGL_RS01260 at 23.774 kb on - strand, within SGL_RS01260 at 23.774 kb on - strand, within SGL_RS01260 at 23.774 kb on - strand, within SGL_RS01260 at 23.823 kb on + strand at 23.854 kb on + strand at 23.860 kb on - strand at 23.902 kb on + strand, within SGL_RS01265 at 23.907 kb on + strand, within SGL_RS01265 at 23.910 kb on - strand, within SGL_RS01265 at 23.910 kb on - strand, within SGL_RS01265 at 23.910 kb on - strand, within SGL_RS01265 at 23.962 kb on - strand, within SGL_RS01265 at 23.974 kb on + strand, within SGL_RS01265 at 23.979 kb on + strand, within SGL_RS01265 at 23.987 kb on - strand, within SGL_RS01265 at 24.000 kb on + strand, within SGL_RS01265 at 24.064 kb on + strand at 24.177 kb on + strand at 24.200 kb on - strand at 24.316 kb on - strand at 24.318 kb on + strand at 24.326 kb on - strand at 24.359 kb on + strand, within SGL_RS01270 at 24.411 kb on + strand, within SGL_RS01270 at 24.421 kb on + strand, within SGL_RS01270 at 24.432 kb on - strand, within SGL_RS01270 at 24.444 kb on + strand, within SGL_RS01270 at 24.526 kb on + strand, within SGL_RS01270 at 24.611 kb on - strand, within SGL_RS01270 at 24.620 kb on - strand, within SGL_RS01270 at 24.752 kb on + strand, within SGL_RS01270 at 24.752 kb on + strand, within SGL_RS01270 at 24.937 kb on + strand at 24.944 kb on + strand at 25.007 kb on + strand at 25.150 kb on - strand at 25.209 kb on - strand at 25.214 kb on - strand at 25.410 kb on - strand, within SGL_RS01275 at 25.478 kb on - strand, within SGL_RS01275 at 25.592 kb on + strand, within SGL_RS01275 at 25.682 kb on - strand, within SGL_RS01275 at 25.720 kb on + strand, within SGL_RS01275 at 25.728 kb on + strand, within SGL_RS01275 at 25.737 kb on - strand, within SGL_RS01275
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation remove 23,774 - SGL_RS01260 0.80 +0.8 23,774 - SGL_RS01260 0.80 +0.9 23,774 - SGL_RS01260 0.80 -0.3 23,774 - SGL_RS01260 0.80 +0.2 23,823 + -0.3 23,854 + -0.2 23,860 - -0.2 23,902 + SGL_RS01265 0.21 +0.3 23,907 + SGL_RS01265 0.23 +0.3 23,910 - SGL_RS01265 0.24 +0.6 23,910 - SGL_RS01265 0.24 +0.8 23,910 - SGL_RS01265 0.24 -0.9 23,962 - SGL_RS01265 0.48 +0.5 23,974 + SGL_RS01265 0.53 +0.2 23,979 + SGL_RS01265 0.55 -0.2 23,987 - SGL_RS01265 0.59 +0.6 24,000 + SGL_RS01265 0.65 -1.6 24,064 + +0.1 24,177 + +0.2 24,200 - -0.8 24,316 - -0.2 24,318 + +0.5 24,326 - -0.7 24,359 + SGL_RS01270 0.11 +0.8 24,411 + SGL_RS01270 0.20 +0.6 24,421 + SGL_RS01270 0.22 +0.4 24,432 - SGL_RS01270 0.24 +0.4 24,444 + SGL_RS01270 0.26 +0.8 24,526 + SGL_RS01270 0.41 +0.9 24,611 - SGL_RS01270 0.56 +0.3 24,620 - SGL_RS01270 0.57 +0.6 24,752 + SGL_RS01270 0.81 +0.8 24,752 + SGL_RS01270 0.81 +0.4 24,937 + +0.3 24,944 + -0.1 25,007 + -1.3 25,150 - +1.3 25,209 - -0.8 25,214 - -0.3 25,410 - SGL_RS01275 0.12 +1.0 25,478 - SGL_RS01275 0.15 +3.5 25,592 + SGL_RS01275 0.20 +0.6 25,682 - SGL_RS01275 0.24 +0.4 25,720 + SGL_RS01275 0.26 -0.4 25,728 + SGL_RS01275 0.26 -0.4 25,737 - SGL_RS01275 0.27 -0.2
Or see this region's nucleotide sequence