Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00345 and SGL_RS00350 are separated by 91 nucleotides SGL_RS00350 and SGL_RS00355 are separated by 120 nucleotides SGL_RS00355 and SGL_RS00360 are separated by 203 nucleotides
SGL_RS00345: SGL_RS00345 - dihydrofolate reductase family protein, at 74,510 to 75,055
_RS00345
SGL_RS00350: SGL_RS00350 - Rpn family recombination-promoting nuclease/putative transposase, at 75,147 to 75,929
_RS00350
SGL_RS00355: SGL_RS00355 - ion channel, at 76,050 to 76,988
_RS00355
SGL_RS00360: SGL_RS00360 - glucose 1-dehydrogenase, at 77,192 to 77,944
_RS00360
Position (kb) 76
77 Strain fitness (log2 ratio) -1
0
1 at 75.461 kb on + strand, within SGL_RS00350 at 75.484 kb on - strand, within SGL_RS00350 at 76.138 kb on - strand at 76.513 kb on + strand, within SGL_RS00355 at 76.523 kb on - strand, within SGL_RS00355 at 76.848 kb on + strand, within SGL_RS00355 at 76.887 kb on - strand, within SGL_RS00355 at 77.124 kb on - strand at 77.149 kb on - strand at 77.173 kb on + strand at 77.185 kb on + strand at 77.193 kb on - strand at 77.213 kb on - strand at 77.355 kb on + strand, within SGL_RS00360 at 77.389 kb on + strand, within SGL_RS00360 at 77.420 kb on + strand, within SGL_RS00360 at 77.428 kb on - strand, within SGL_RS00360 at 77.428 kb on - strand, within SGL_RS00360 at 77.576 kb on + strand, within SGL_RS00360 at 77.827 kb on - strand, within SGL_RS00360 at 77.852 kb on + strand, within SGL_RS00360 at 77.987 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with air enriched with 5% CO2 but diluted to prevent light attenuation remove 75,461 + SGL_RS00350 0.40 +0.9 75,484 - SGL_RS00350 0.43 -0.0 76,138 - -0.5 76,513 + SGL_RS00355 0.49 -0.9 76,523 - SGL_RS00355 0.50 -0.2 76,848 + SGL_RS00355 0.85 -0.2 76,887 - SGL_RS00355 0.89 -0.4 77,124 - -1.0 77,149 - -1.1 77,173 + -0.9 77,185 + +0.4 77,193 - -1.1 77,213 - -0.6 77,355 + SGL_RS00360 0.22 +0.6 77,389 + SGL_RS00360 0.26 -0.1 77,420 + SGL_RS00360 0.30 +1.4 77,428 - SGL_RS00360 0.31 +0.2 77,428 - SGL_RS00360 0.31 +0.1 77,576 + SGL_RS00360 0.51 -0.3 77,827 - SGL_RS00360 0.84 -0.2 77,852 + SGL_RS00360 0.88 +0.4 77,987 + -0.2
Or see this region's nucleotide sequence