Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18280 and SGL_RS18285 are separated by 138 nucleotides SGL_RS18285 and SGL_RS18290 are separated by 74 nucleotides SGL_RS18290 and SGL_RS18295 are separated by 142 nucleotides
SGL_RS18280: SGL_RS18280 - recombinase RecA, at 3,549,956 to 3,551,020
_RS18280
SGL_RS18285: SGL_RS18285 - ABC transporter permease, at 3,551,159 to 3,552,382
_RS18285
SGL_RS18290: SGL_RS18290 - transcriptional repressor, at 3,552,457 to 3,552,954
_RS18290
SGL_RS18295: SGL_RS18295 - creatininase family protein, at 3,553,097 to 3,553,918
_RS18295
Position (kb)
3551
3552
3553 Strain fitness (log2 ratio)
-1
0
1 at 3551.098 kb on - strand at 3551.098 kb on - strand at 3551.200 kb on + strand at 3551.254 kb on - strand at 3551.294 kb on + strand, within SGL_RS18285 at 3551.302 kb on - strand, within SGL_RS18285 at 3551.302 kb on - strand, within SGL_RS18285 at 3551.302 kb on - strand, within SGL_RS18285 at 3551.302 kb on - strand, within SGL_RS18285 at 3551.331 kb on + strand, within SGL_RS18285 at 3551.423 kb on - strand, within SGL_RS18285 at 3551.437 kb on + strand, within SGL_RS18285 at 3551.478 kb on + strand, within SGL_RS18285 at 3551.478 kb on + strand, within SGL_RS18285 at 3551.604 kb on - strand, within SGL_RS18285 at 3551.700 kb on + strand, within SGL_RS18285 at 3551.738 kb on + strand, within SGL_RS18285 at 3551.741 kb on - strand, within SGL_RS18285 at 3551.907 kb on - strand, within SGL_RS18285 at 3552.131 kb on + strand, within SGL_RS18285 at 3552.167 kb on + strand, within SGL_RS18285 at 3552.191 kb on - strand, within SGL_RS18285 at 3552.366 kb on - strand at 3552.370 kb on + strand at 3552.370 kb on + strand at 3553.156 kb on + strand at 3553.351 kb on + strand, within SGL_RS18295 at 3553.366 kb on - strand, within SGL_RS18295
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air but diluted to prevent light attenuation remove 3,551,098 - +0.3 3,551,098 - +0.1 3,551,200 + -0.2 3,551,254 - -1.2 3,551,294 + SGL_RS18285 0.11 -0.4 3,551,302 - SGL_RS18285 0.12 -0.5 3,551,302 - SGL_RS18285 0.12 -1.1 3,551,302 - SGL_RS18285 0.12 +1.2 3,551,302 - SGL_RS18285 0.12 -0.8 3,551,331 + SGL_RS18285 0.14 -0.4 3,551,423 - SGL_RS18285 0.22 -1.5 3,551,437 + SGL_RS18285 0.23 -0.9 3,551,478 + SGL_RS18285 0.26 -0.5 3,551,478 + SGL_RS18285 0.26 -1.0 3,551,604 - SGL_RS18285 0.36 -1.5 3,551,700 + SGL_RS18285 0.44 -0.9 3,551,738 + SGL_RS18285 0.47 -0.4 3,551,741 - SGL_RS18285 0.48 -1.4 3,551,907 - SGL_RS18285 0.61 +0.0 3,552,131 + SGL_RS18285 0.79 -0.9 3,552,167 + SGL_RS18285 0.82 -1.3 3,552,191 - SGL_RS18285 0.84 -1.4 3,552,366 - -0.4 3,552,370 + -0.9 3,552,370 + -0.5 3,553,156 + -0.2 3,553,351 + SGL_RS18295 0.31 +0.1 3,553,366 - SGL_RS18295 0.33 +0.2
Or see this region's nucleotide sequence