Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09510 and SGL_RS09515 overlap by 4 nucleotides SGL_RS09515 and SGL_RS09520 are separated by 13 nucleotides SGL_RS09520 and SGL_RS09525 overlap by 11 nucleotides SGL_RS09525 and SGL_RS09530 are separated by 30 nucleotides
SGL_RS09510: SGL_RS09510 - beta-3-deoxy-D-manno-oct-2-ulosonic acid transferase, at 1,628,045 to 1,629,607
_RS09510
SGL_RS09515: SGL_RS09515 - glycosyltransferase family 2 protein, at 1,629,604 to 1,630,434
_RS09515
SGL_RS09520: SGL_RS09520 - DUF115 domain-containing protein, at 1,630,448 to 1,631,005
_RS09520
SGL_RS09525: SGL_RS09525 - hypothetical protein, at 1,630,995 to 1,631,240
_RS09525
SGL_RS09530: SGL_RS09530 - glycosyltransferase, at 1,631,271 to 1,632,530
_RS09530
Position (kb) 1630
1631
1632 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1629.486 kb on + strand at 1629.828 kb on - strand, within SGL_RS09515 at 1630.927 kb on + strand, within SGL_RS09520 at 1630.934 kb on - strand, within SGL_RS09520 at 1631.764 kb on + strand, within SGL_RS09530 at 1631.964 kb on + strand, within SGL_RS09530
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air but diluted to prevent light attenuation remove 1,629,486 + -1.3 1,629,828 - SGL_RS09515 0.27 -0.7 1,630,927 + SGL_RS09520 0.86 -1.5 1,630,934 - SGL_RS09520 0.87 -2.9 1,631,764 + SGL_RS09530 0.39 -5.4 1,631,964 + SGL_RS09530 0.55 -4.9
Or see this region's nucleotide sequence