Strain Fitness in Synechocystis sp000284455 PCC 6803 around SGL_RS09505

Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air but diluted to prevent light attenuation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSGL_RS09490 and SGL_RS19865 are separated by 69 nucleotidesSGL_RS19865 and SGL_RS18860 overlap by 91 nucleotidesSGL_RS18860 and SGL_RS09505 overlap by 32 nucleotidesSGL_RS09505 and SGL_RS09510 are separated by 26 nucleotides SGL_RS09490: SGL_RS09490 - CBS domain-containing protein, at 1,625,622 to 1,626,095 _RS09490 SGL_RS19865: SGL_RS19865 - IS630 transposase-related protein, at 1,626,165 to 1,626,524 _RS19865 SGL_RS18860: SGL_RS18860 - IS630 family transposase, at 1,626,434 to 1,627,012 _RS18860 SGL_RS09505: SGL_RS09505 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 1,626,981 to 1,628,018 _RS09505 SGL_RS09510: SGL_RS09510 - beta-3-deoxy-D-manno-oct-2-ulosonic acid transferase, at 1,628,045 to 1,629,607 _RS09510 Position (kb) 1626 1627 1628 1629Strain fitness (log2 ratio) -3 -2 -1 0 1at 1626.001 kb on - strand, within SGL_RS09490at 1626.001 kb on - strand, within SGL_RS09490at 1627.100 kb on + strand, within SGL_RS09505at 1627.108 kb on - strand, within SGL_RS09505at 1627.126 kb on - strand, within SGL_RS09505at 1627.175 kb on + strand, within SGL_RS09505at 1628.372 kb on - strand, within SGL_RS09510

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Per-strain Table

Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air but diluted to prevent light attenuation
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1,626,001 - SGL_RS09490 0.80 -0.3
1,626,001 - SGL_RS09490 0.80 -0.3
1,627,100 + SGL_RS09505 0.11 -2.9
1,627,108 - SGL_RS09505 0.12 -0.2
1,627,126 - SGL_RS09505 0.14 +0.5
1,627,175 + SGL_RS09505 0.19 -0.4
1,628,372 - SGL_RS09510 0.21 -0.6

Or see this region's nucleotide sequence