Experiment: growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08995 and SGL_RS09000 are separated by 86 nucleotides SGL_RS09000 and SGL_RS09005 are separated by 583 nucleotides SGL_RS09005 and SGL_RS09010 are separated by 118 nucleotides
SGL_RS08995: SGL_RS08995 - NblA/ycf18 family protein, at 1,523,527 to 1,523,709
_RS08995
SGL_RS09000: SGL_RS09000 - NblA/ycf18 family protein, at 1,523,796 to 1,523,984
_RS09000
SGL_RS09005: SGL_RS09005 - hypothetical protein, at 1,524,568 to 1,525,086
_RS09005
SGL_RS09010: SGL_RS09010 - zinc ribbon domain-containing protein, at 1,525,205 to 1,526,182
_RS09010
Position (kb)
1524
1525
1526 Strain fitness (log2 ratio)
-1
0
1 at 1523.634 kb on - strand, within SGL_RS08995 at 1523.685 kb on + strand, within SGL_RS08995 at 1523.685 kb on + strand, within SGL_RS08995 at 1523.693 kb on - strand at 1523.806 kb on + strand at 1523.897 kb on + strand, within SGL_RS09000 at 1524.101 kb on + strand at 1524.302 kb on + strand at 1524.663 kb on + strand, within SGL_RS09005 at 1524.786 kb on - strand, within SGL_RS09005 at 1524.804 kb on + strand, within SGL_RS09005 at 1524.804 kb on + strand, within SGL_RS09005 at 1524.889 kb on + strand, within SGL_RS09005 at 1524.952 kb on + strand, within SGL_RS09005 at 1524.960 kb on - strand, within SGL_RS09005 at 1525.257 kb on - strand at 1525.289 kb on + strand at 1525.296 kb on + strand at 1525.315 kb on - strand, within SGL_RS09010 at 1525.356 kb on + strand, within SGL_RS09010 at 1525.364 kb on - strand, within SGL_RS09010 at 1525.746 kb on + strand, within SGL_RS09010 at 1525.761 kb on + strand, within SGL_RS09010 at 1525.952 kb on + strand, within SGL_RS09010 at 1525.953 kb on + strand, within SGL_RS09010 at 1526.009 kb on - strand, within SGL_RS09010
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 TES pH8 and bubbled with ambient air but diluted to prevent light attenuation remove 1,523,634 - SGL_RS08995 0.58 +0.2 1,523,685 + SGL_RS08995 0.86 -0.1 1,523,685 + SGL_RS08995 0.86 -0.1 1,523,693 - -1.5 1,523,806 + +0.1 1,523,897 + SGL_RS09000 0.53 +0.1 1,524,101 + +0.4 1,524,302 + -0.4 1,524,663 + SGL_RS09005 0.18 +0.0 1,524,786 - SGL_RS09005 0.42 -0.7 1,524,804 + SGL_RS09005 0.45 -0.4 1,524,804 + SGL_RS09005 0.45 -0.8 1,524,889 + SGL_RS09005 0.62 -0.6 1,524,952 + SGL_RS09005 0.74 +0.1 1,524,960 - SGL_RS09005 0.76 +0.4 1,525,257 - +0.3 1,525,289 + +0.3 1,525,296 + -0.3 1,525,315 - SGL_RS09010 0.11 -0.7 1,525,356 + SGL_RS09010 0.15 +0.0 1,525,364 - SGL_RS09010 0.16 +0.2 1,525,746 + SGL_RS09010 0.55 -0.8 1,525,761 + SGL_RS09010 0.57 -0.3 1,525,952 + SGL_RS09010 0.76 +0.6 1,525,953 + SGL_RS09010 0.76 +0.3 1,526,009 - SGL_RS09010 0.82 -0.1
Or see this region's nucleotide sequence