Experiment: growth in full media bubbled with air enriched in 1% CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16725 and SGL_RS20185 are separated by 72 nucleotides SGL_RS20185 and SGL_RS16730 are separated by 0 nucleotides SGL_RS16730 and SGL_RS16735 are separated by 79 nucleotides SGL_RS16735 and SGL_RS16740 are separated by 77 nucleotides
SGL_RS16725: SGL_RS16725 - glycosyltransferase GtrB, at 3,210,650 to 3,211,606
_RS16725
SGL_RS20185: SGL_RS20185 - hypothetical protein, at 3,211,679 to 3,211,807
_RS20185
SGL_RS16730: SGL_RS16730 - gamma-glutamyl-gamma-aminobutyrate hydrolase family protein, at 3,211,808 to 3,212,518
_RS16730
SGL_RS16735: SGL_RS16735 - ATP-dependent Clp endopeptidase proteolytic subunit ClpP, at 3,212,598 to 3,213,194
_RS16735
SGL_RS16740: SGL_RS16740 - tryptophan synthase subunit beta, at 3,213,272 to 3,214,510
_RS16740
Position (kb)
3212
3213
3214 Strain fitness (log2 ratio)
-1
0
1 at 3211.766 kb on - strand, within SGL_RS20185 at 3211.879 kb on + strand, within SGL_RS16730 at 3211.966 kb on - strand, within SGL_RS16730 at 3212.009 kb on + strand, within SGL_RS16730 at 3212.084 kb on + strand, within SGL_RS16730 at 3212.164 kb on + strand, within SGL_RS16730 at 3212.359 kb on + strand, within SGL_RS16730 at 3212.361 kb on - strand, within SGL_RS16730 at 3212.495 kb on - strand at 3212.689 kb on + strand, within SGL_RS16735 at 3212.964 kb on + strand, within SGL_RS16735 at 3212.964 kb on + strand, within SGL_RS16735 at 3212.964 kb on + strand, within SGL_RS16735 at 3212.976 kb on + strand, within SGL_RS16735 at 3212.984 kb on - strand, within SGL_RS16735 at 3214.157 kb on + strand, within SGL_RS16740
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 remove 3,211,766 - SGL_RS20185 0.67 -0.5 3,211,879 + SGL_RS16730 0.10 -0.4 3,211,966 - SGL_RS16730 0.22 -0.1 3,212,009 + SGL_RS16730 0.28 -1.5 3,212,084 + SGL_RS16730 0.39 -1.0 3,212,164 + SGL_RS16730 0.50 -0.7 3,212,359 + SGL_RS16730 0.77 -0.8 3,212,361 - SGL_RS16730 0.78 -0.0 3,212,495 - -0.0 3,212,689 + SGL_RS16735 0.15 +0.8 3,212,964 + SGL_RS16735 0.61 -1.2 3,212,964 + SGL_RS16735 0.61 -0.8 3,212,964 + SGL_RS16735 0.61 -0.2 3,212,976 + SGL_RS16735 0.63 -0.2 3,212,984 - SGL_RS16735 0.65 -1.5 3,214,157 + SGL_RS16740 0.71 -1.2
Or see this region's nucleotide sequence