Experiment: growth in full media bubbled with air enriched in 1% CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14425 and SGL_RS14430 overlap by 79 nucleotides SGL_RS14430 and SGL_RS14435 are separated by 2 nucleotides SGL_RS14435 and SGL_RS14440 are separated by 0 nucleotides SGL_RS14440 and SGL_RS14445 are separated by 5 nucleotides
SGL_RS14425: SGL_RS14425 - energy-coupling factor ABC transporter ATP-binding protein, at 2,691,528 to 2,692,382
_RS14425
SGL_RS14430: SGL_RS14430 - cobalt ECF transporter T component CbiQ, at 2,692,304 to 2,693,077
_RS14430
SGL_RS14435: SGL_RS14435 - cobalt transporter CbiM, at 2,693,080 to 2,693,724
_RS14435
SGL_RS14440: SGL_RS14440 - carboxypeptidase-like regulatory domain-containing protein, at 2,693,725 to 2,694,255
_RS14440
SGL_RS14445: SGL_RS14445 - hypothetical protein, at 2,694,261 to 2,695,145
_RS14445
Position (kb)
2693
2694 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2692.146 kb on + strand, within SGL_RS14425 at 2692.220 kb on - strand, within SGL_RS14425 at 2692.317 kb on - strand at 2692.364 kb on + strand at 2692.372 kb on - strand at 2692.440 kb on + strand, within SGL_RS14430 at 2692.440 kb on + strand, within SGL_RS14430 at 2692.468 kb on + strand, within SGL_RS14430 at 2692.476 kb on - strand, within SGL_RS14430 at 2692.476 kb on - strand, within SGL_RS14430 at 2692.543 kb on + strand, within SGL_RS14430 at 2692.551 kb on - strand, within SGL_RS14430 at 2692.564 kb on - strand, within SGL_RS14430 at 2692.645 kb on - strand, within SGL_RS14430 at 2692.970 kb on + strand, within SGL_RS14430 at 2693.046 kb on + strand at 2693.067 kb on + strand at 2693.437 kb on + strand, within SGL_RS14435 at 2693.506 kb on - strand, within SGL_RS14435 at 2693.737 kb on - strand at 2693.737 kb on - strand at 2693.920 kb on - strand, within SGL_RS14440 at 2693.953 kb on - strand, within SGL_RS14440 at 2694.412 kb on - strand, within SGL_RS14445 at 2694.423 kb on + strand, within SGL_RS14445 at 2694.628 kb on + strand, within SGL_RS14445 at 2694.678 kb on + strand, within SGL_RS14445 at 2694.678 kb on + strand, within SGL_RS14445 at 2694.686 kb on - strand, within SGL_RS14445
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 remove 2,692,146 + SGL_RS14425 0.72 -2.3 2,692,220 - SGL_RS14425 0.81 -2.6 2,692,317 - -0.2 2,692,364 + -3.3 2,692,372 - -0.2 2,692,440 + SGL_RS14430 0.18 -1.2 2,692,440 + SGL_RS14430 0.18 -1.5 2,692,468 + SGL_RS14430 0.21 -0.2 2,692,476 - SGL_RS14430 0.22 -2.0 2,692,476 - SGL_RS14430 0.22 -2.0 2,692,543 + SGL_RS14430 0.31 -1.6 2,692,551 - SGL_RS14430 0.32 -4.0 2,692,564 - SGL_RS14430 0.34 -2.6 2,692,645 - SGL_RS14430 0.44 -0.8 2,692,970 + SGL_RS14430 0.86 -0.8 2,693,046 + -1.8 2,693,067 + -2.5 2,693,437 + SGL_RS14435 0.55 -2.6 2,693,506 - SGL_RS14435 0.66 -2.8 2,693,737 - -4.4 2,693,737 - -0.2 2,693,920 - SGL_RS14440 0.37 -1.8 2,693,953 - SGL_RS14440 0.43 -0.2 2,694,412 - SGL_RS14445 0.17 -3.7 2,694,423 + SGL_RS14445 0.18 -1.2 2,694,628 + SGL_RS14445 0.41 -0.8 2,694,678 + SGL_RS14445 0.47 -0.2 2,694,678 + SGL_RS14445 0.47 -0.2 2,694,686 - SGL_RS14445 0.48 +0.8
Or see this region's nucleotide sequence