Experiment: growth in full media bubbled with air enriched in 1% CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04385 and SGL_RS04390 are separated by 157 nucleotides SGL_RS04390 and SGL_RS04395 overlap by 4 nucleotides SGL_RS04395 and SGL_RS04400 are separated by 2 nucleotides
SGL_RS04385: SGL_RS04385 - glutamate decarboxylase, at 543,468 to 544,871
_RS04385
SGL_RS04390: SGL_RS04390 - DUF433 domain-containing protein, at 545,029 to 545,253
_RS04390
SGL_RS04395: SGL_RS04395 - DUF5615 family PIN-like protein, at 545,250 to 545,588
_RS04395
SGL_RS04400: SGL_RS04400 - carbon-nitrogen hydrolase family protein, at 545,591 to 546,556
_RS04400
Position (kb) 545
546 Strain fitness (log2 ratio) -2
-1
0
1 at 544.065 kb on - strand, within SGL_RS04385 at 544.160 kb on - strand, within SGL_RS04385 at 544.228 kb on - strand, within SGL_RS04385 at 544.304 kb on + strand, within SGL_RS04385 at 544.522 kb on - strand, within SGL_RS04385 at 544.662 kb on + strand, within SGL_RS04385 at 544.794 kb on + strand at 544.842 kb on - strand at 545.078 kb on - strand, within SGL_RS04390 at 545.194 kb on + strand, within SGL_RS04390 at 545.211 kb on - strand, within SGL_RS04390 at 545.353 kb on + strand, within SGL_RS04395 at 545.388 kb on + strand, within SGL_RS04395 at 545.516 kb on + strand, within SGL_RS04395 at 545.546 kb on + strand, within SGL_RS04395 at 545.546 kb on + strand, within SGL_RS04395 at 545.613 kb on + strand at 545.621 kb on - strand at 545.672 kb on + strand at 545.672 kb on + strand at 545.792 kb on - strand, within SGL_RS04400 at 545.885 kb on - strand, within SGL_RS04400 at 545.896 kb on + strand, within SGL_RS04400 at 545.997 kb on - strand, within SGL_RS04400
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 remove 544,065 - SGL_RS04385 0.43 +0.3 544,160 - SGL_RS04385 0.49 +0.1 544,228 - SGL_RS04385 0.54 +0.0 544,304 + SGL_RS04385 0.60 +0.2 544,522 - SGL_RS04385 0.75 -0.2 544,662 + SGL_RS04385 0.85 +0.2 544,794 + -0.3 544,842 - -0.1 545,078 - SGL_RS04390 0.22 -2.3 545,194 + SGL_RS04390 0.73 -2.7 545,211 - SGL_RS04390 0.81 -1.3 545,353 + SGL_RS04395 0.30 +0.5 545,388 + SGL_RS04395 0.41 -0.4 545,516 + SGL_RS04395 0.78 +0.2 545,546 + SGL_RS04395 0.87 +1.6 545,546 + SGL_RS04395 0.87 +0.4 545,613 + -0.1 545,621 - +0.4 545,672 + +0.3 545,672 + -0.2 545,792 - SGL_RS04400 0.21 -0.1 545,885 - SGL_RS04400 0.30 +0.1 545,896 + SGL_RS04400 0.32 +0.1 545,997 - SGL_RS04400 0.42 +0.3
Or see this region's nucleotide sequence