Experiment: growth in full media bubbled with air enriched in 1% CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04330 and SGL_RS04335 are separated by 28 nucleotides SGL_RS04335 and SGL_RS04340 are separated by 210 nucleotides
SGL_RS04330: SGL_RS04330 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 529,735 to 530,688
_RS04330
SGL_RS04335: SGL_RS04335 - GNAT family N-acetyltransferase, at 530,717 to 531,235
_RS04335
SGL_RS04340: SGL_RS04340 - type I glutamate--ammonia ligase, at 531,446 to 532,867
_RS04340
Position (kb) 530
531
532 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 529.761 kb on + strand at 530.141 kb on - strand, within SGL_RS04330 at 530.518 kb on - strand, within SGL_RS04330 at 530.806 kb on + strand, within SGL_RS04335 at 530.806 kb on + strand, within SGL_RS04335 at 530.814 kb on - strand, within SGL_RS04335 at 530.895 kb on + strand, within SGL_RS04335 at 530.966 kb on + strand, within SGL_RS04335 at 530.974 kb on + strand, within SGL_RS04335 at 531.193 kb on - strand at 531.337 kb on - strand at 531.395 kb on + strand at 531.493 kb on + strand at 531.539 kb on - strand at 531.575 kb on + strand at 531.585 kb on - strand at 531.620 kb on - strand, within SGL_RS04340 at 531.664 kb on + strand, within SGL_RS04340 at 531.751 kb on + strand, within SGL_RS04340 at 531.751 kb on + strand, within SGL_RS04340 at 531.759 kb on - strand, within SGL_RS04340 at 531.764 kb on - strand, within SGL_RS04340 at 531.764 kb on - strand, within SGL_RS04340 at 531.779 kb on - strand, within SGL_RS04340 at 531.914 kb on + strand, within SGL_RS04340 at 531.960 kb on - strand, within SGL_RS04340 at 532.006 kb on + strand, within SGL_RS04340 at 532.093 kb on + strand, within SGL_RS04340 at 532.102 kb on - strand, within SGL_RS04340
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 remove 529,761 + -3.0 530,141 - SGL_RS04330 0.43 -0.3 530,518 - SGL_RS04330 0.82 +0.1 530,806 + SGL_RS04335 0.17 -2.0 530,806 + SGL_RS04335 0.17 -3.4 530,814 - SGL_RS04335 0.19 -2.2 530,895 + SGL_RS04335 0.34 +0.7 530,966 + SGL_RS04335 0.48 -2.6 530,974 + SGL_RS04335 0.50 -2.6 531,193 - -2.7 531,337 - -0.0 531,395 + +0.3 531,493 + -0.6 531,539 - +0.1 531,575 + -0.6 531,585 - -0.4 531,620 - SGL_RS04340 0.12 +0.2 531,664 + SGL_RS04340 0.15 +0.6 531,751 + SGL_RS04340 0.21 +0.2 531,751 + SGL_RS04340 0.21 -0.0 531,759 - SGL_RS04340 0.22 -0.3 531,764 - SGL_RS04340 0.22 -0.2 531,764 - SGL_RS04340 0.22 -0.3 531,779 - SGL_RS04340 0.23 -0.3 531,914 + SGL_RS04340 0.33 -0.3 531,960 - SGL_RS04340 0.36 -0.5 532,006 + SGL_RS04340 0.39 -0.3 532,093 + SGL_RS04340 0.45 -0.6 532,102 - SGL_RS04340 0.46 -0.7
Or see this region's nucleotide sequence