Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17690 and SGL_RS17695 are separated by 18 nucleotides SGL_RS17695 and SGL_RS17700 are separated by 13 nucleotides SGL_RS17700 and SGL_RS17705 are separated by 255 nucleotides SGL_RS17705 and SGL_RS17710 overlap by 8 nucleotides
SGL_RS17690: SGL_RS17690 - hypothetical protein, at 3,424,394 to 3,424,648
_RS17690
SGL_RS17695: SGL_RS17695 - DNA adenine methylase, at 3,424,667 to 3,425,524
_RS17695
SGL_RS17700: SGL_RS17700 - acetyl-CoA carboxylase carboxyl transferase subunit alpha, at 3,425,538 to 3,426,518
_RS17700
SGL_RS17705: SGL_RS17705 - type II toxin-antitoxin system HicA family toxin, at 3,426,774 to 3,427,001
_RS17705
SGL_RS17710: SGL_RS17710 - type II toxin-antitoxin system HicB family antitoxin, at 3,426,994 to 3,427,203
_RS17710
Position (kb)
3425
3426
3427 Strain fitness (log2 ratio)
-2
-1
0
1 at 3424.625 kb on + strand at 3424.858 kb on + strand, within SGL_RS17695 at 3426.631 kb on - strand at 3426.695 kb on - strand at 3426.746 kb on - strand at 3427.006 kb on - strand at 3427.278 kb on + strand at 3427.278 kb on + strand at 3427.302 kb on + strand at 3427.310 kb on - strand at 3427.343 kb on + strand at 3427.367 kb on + strand at 3427.430 kb on - strand at 3427.444 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 3,424,625 + +0.2 3,424,858 + SGL_RS17695 0.22 -2.6 3,426,631 - -0.4 3,426,695 - +0.6 3,426,746 - +0.1 3,427,006 - -0.7 3,427,278 + -0.9 3,427,278 + -0.3 3,427,302 + -0.1 3,427,310 - +0.2 3,427,343 + +0.1 3,427,367 + -0.4 3,427,430 - -0.1 3,427,444 + +0.2
Or see this region's nucleotide sequence