Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16525 and SGL_RS16530 are separated by 71 nucleotides SGL_RS16530 and SGL_RS16535 are separated by 12 nucleotides SGL_RS16535 and SGL_RS16540 are separated by 62 nucleotides
SGL_RS16525: SGL_RS16525 - methylglyoxal synthase, at 3,159,350 to 3,160,651
_RS16525
SGL_RS16530: SGL_RS16530 - pyridoxal phosphate-dependent aminotransferase, at 3,160,723 to 3,161,886
_RS16530
SGL_RS16535: SGL_RS16535 - D-alanyl-D-alanine carboxypeptidase family protein, at 3,161,899 to 3,162,675
_RS16535
SGL_RS16540: SGL_RS16540 - carotene isomerase, at 3,162,738 to 3,164,243
_RS16540
Position (kb)
3160
3161
3162 Strain fitness (log2 ratio)
-1
0
1 at 3159.755 kb on - strand, within SGL_RS16525 at 3159.843 kb on - strand, within SGL_RS16525 at 3159.852 kb on + strand, within SGL_RS16525 at 3159.860 kb on - strand, within SGL_RS16525 at 3159.873 kb on + strand, within SGL_RS16525 at 3159.873 kb on + strand, within SGL_RS16525 at 3159.881 kb on - strand, within SGL_RS16525 at 3159.881 kb on - strand, within SGL_RS16525 at 3159.881 kb on - strand, within SGL_RS16525 at 3159.881 kb on - strand, within SGL_RS16525 at 3159.881 kb on - strand, within SGL_RS16525 at 3159.881 kb on - strand, within SGL_RS16525 at 3159.881 kb on - strand, within SGL_RS16525 at 3159.883 kb on + strand, within SGL_RS16525 at 3159.976 kb on + strand, within SGL_RS16525 at 3159.978 kb on + strand, within SGL_RS16525 at 3159.978 kb on + strand, within SGL_RS16525 at 3160.033 kb on - strand, within SGL_RS16525 at 3160.233 kb on + strand, within SGL_RS16525 at 3160.238 kb on + strand, within SGL_RS16525 at 3160.338 kb on + strand, within SGL_RS16525 at 3160.340 kb on + strand, within SGL_RS16525 at 3160.343 kb on + strand, within SGL_RS16525 at 3160.357 kb on + strand, within SGL_RS16525 at 3160.357 kb on + strand, within SGL_RS16525 at 3160.428 kb on + strand, within SGL_RS16525 at 3160.641 kb on - strand at 3160.766 kb on - strand at 3160.775 kb on + strand at 3160.826 kb on - strand at 3160.827 kb on - strand at 3160.857 kb on + strand, within SGL_RS16530 at 3161.011 kb on - strand, within SGL_RS16530 at 3161.028 kb on + strand, within SGL_RS16530 at 3161.046 kb on - strand, within SGL_RS16530 at 3161.130 kb on + strand, within SGL_RS16530 at 3161.187 kb on + strand, within SGL_RS16530 at 3161.318 kb on - strand, within SGL_RS16530 at 3161.379 kb on + strand, within SGL_RS16530 at 3161.412 kb on - strand, within SGL_RS16530 at 3161.430 kb on - strand, within SGL_RS16530 at 3161.430 kb on - strand, within SGL_RS16530 at 3161.501 kb on + strand, within SGL_RS16530 at 3161.647 kb on - strand, within SGL_RS16530 at 3161.682 kb on - strand, within SGL_RS16530 at 3161.776 kb on - strand at 3161.804 kb on + strand at 3161.855 kb on + strand at 3162.166 kb on + strand, within SGL_RS16535 at 3162.407 kb on - strand, within SGL_RS16535 at 3162.474 kb on + strand, within SGL_RS16535 at 3162.491 kb on + strand, within SGL_RS16535 at 3162.491 kb on + strand, within SGL_RS16535 at 3162.499 kb on - strand, within SGL_RS16535 at 3162.567 kb on + strand, within SGL_RS16535 at 3162.574 kb on + strand, within SGL_RS16535 at 3162.575 kb on - strand, within SGL_RS16535 at 3162.801 kb on + strand at 3162.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 3,159,755 - SGL_RS16525 0.31 -0.3 3,159,843 - SGL_RS16525 0.38 -0.6 3,159,852 + SGL_RS16525 0.39 +0.3 3,159,860 - SGL_RS16525 0.39 +0.6 3,159,873 + SGL_RS16525 0.40 -0.4 3,159,873 + SGL_RS16525 0.40 -0.4 3,159,881 - SGL_RS16525 0.41 -0.3 3,159,881 - SGL_RS16525 0.41 -0.4 3,159,881 - SGL_RS16525 0.41 -0.4 3,159,881 - SGL_RS16525 0.41 +0.1 3,159,881 - SGL_RS16525 0.41 -0.6 3,159,881 - SGL_RS16525 0.41 -0.0 3,159,881 - SGL_RS16525 0.41 -0.2 3,159,883 + SGL_RS16525 0.41 +0.2 3,159,976 + SGL_RS16525 0.48 -0.1 3,159,978 + SGL_RS16525 0.48 -0.4 3,159,978 + SGL_RS16525 0.48 -0.2 3,160,033 - SGL_RS16525 0.52 -0.4 3,160,233 + SGL_RS16525 0.68 +0.2 3,160,238 + SGL_RS16525 0.68 -0.3 3,160,338 + SGL_RS16525 0.76 -0.5 3,160,340 + SGL_RS16525 0.76 -0.3 3,160,343 + SGL_RS16525 0.76 -0.5 3,160,357 + SGL_RS16525 0.77 -0.3 3,160,357 + SGL_RS16525 0.77 +0.2 3,160,428 + SGL_RS16525 0.83 -0.0 3,160,641 - +0.1 3,160,766 - -0.6 3,160,775 + +0.3 3,160,826 - +0.2 3,160,827 - +0.4 3,160,857 + SGL_RS16530 0.12 +0.3 3,161,011 - SGL_RS16530 0.25 -0.0 3,161,028 + SGL_RS16530 0.26 -0.6 3,161,046 - SGL_RS16530 0.28 -0.2 3,161,130 + SGL_RS16530 0.35 +1.3 3,161,187 + SGL_RS16530 0.40 -1.6 3,161,318 - SGL_RS16530 0.51 -0.1 3,161,379 + SGL_RS16530 0.56 +0.2 3,161,412 - SGL_RS16530 0.59 -0.4 3,161,430 - SGL_RS16530 0.61 -0.2 3,161,430 - SGL_RS16530 0.61 +0.3 3,161,501 + SGL_RS16530 0.67 +0.2 3,161,647 - SGL_RS16530 0.79 -0.7 3,161,682 - SGL_RS16530 0.82 +0.2 3,161,776 - -0.1 3,161,804 + +0.5 3,161,855 + +0.7 3,162,166 + SGL_RS16535 0.34 +1.0 3,162,407 - SGL_RS16535 0.65 +0.3 3,162,474 + SGL_RS16535 0.74 +0.6 3,162,491 + SGL_RS16535 0.76 +0.2 3,162,491 + SGL_RS16535 0.76 +0.3 3,162,499 - SGL_RS16535 0.77 -0.2 3,162,567 + SGL_RS16535 0.86 -0.0 3,162,574 + SGL_RS16535 0.87 +0.9 3,162,575 - SGL_RS16535 0.87 +0.2 3,162,801 + +0.8 3,162,809 - -0.1
Or see this region's nucleotide sequence