Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15625 and SGL_RS15630 overlap by 29 nucleotides SGL_RS15630 and SGL_RS15635 are separated by 114 nucleotides SGL_RS15635 and SGL_RS15640 are separated by 134 nucleotides SGL_RS15640 and SGL_RS15645 are separated by 42 nucleotides
SGL_RS15625: SGL_RS15625 - amidohydrolase family protein, at 2,956,107 to 2,957,153
_RS15625
SGL_RS15630: SGL_RS15630 - 2Fe-2S iron-sulfur cluster-binding protein, at 2,957,125 to 2,957,364
_RS15630
SGL_RS15635: SGL_RS15635 - hypothetical protein, at 2,957,479 to 2,957,901
_RS15635
SGL_RS15640: SGL_RS15640 - pyruvate kinase, at 2,958,036 to 2,959,487
_RS15640
SGL_RS15645: SGL_RS15645 - NAD(P)/FAD-dependent oxidoreductase, at 2,959,530 to 2,960,756
_RS15645
Position (kb)
2958
2959
2960 Strain fitness (log2 ratio)
-1
0
1 at 2957.119 kb on + strand at 2957.169 kb on + strand, within SGL_RS15630 at 2957.177 kb on - strand, within SGL_RS15630 at 2957.178 kb on - strand, within SGL_RS15630 at 2957.196 kb on - strand, within SGL_RS15630 at 2957.199 kb on + strand, within SGL_RS15630 at 2957.242 kb on - strand, within SGL_RS15630 at 2957.242 kb on - strand, within SGL_RS15630 at 2957.443 kb on + strand at 2957.453 kb on + strand at 2957.524 kb on + strand, within SGL_RS15635 at 2957.526 kb on + strand, within SGL_RS15635 at 2957.599 kb on + strand, within SGL_RS15635 at 2957.599 kb on + strand, within SGL_RS15635 at 2957.742 kb on - strand, within SGL_RS15635 at 2957.793 kb on - strand, within SGL_RS15635 at 2957.979 kb on - strand at 2958.000 kb on - strand at 2958.159 kb on - strand at 2958.429 kb on + strand, within SGL_RS15640 at 2958.437 kb on - strand, within SGL_RS15640 at 2958.535 kb on - strand, within SGL_RS15640 at 2958.627 kb on - strand, within SGL_RS15640 at 2958.636 kb on + strand, within SGL_RS15640 at 2958.652 kb on - strand, within SGL_RS15640 at 2958.728 kb on - strand, within SGL_RS15640 at 2958.733 kb on - strand, within SGL_RS15640 at 2958.824 kb on - strand, within SGL_RS15640 at 2958.844 kb on - strand, within SGL_RS15640 at 2958.844 kb on - strand, within SGL_RS15640 at 2958.850 kb on - strand, within SGL_RS15640 at 2958.852 kb on + strand, within SGL_RS15640 at 2958.953 kb on - strand, within SGL_RS15640 at 2958.964 kb on - strand, within SGL_RS15640 at 2958.996 kb on - strand, within SGL_RS15640 at 2959.101 kb on - strand, within SGL_RS15640 at 2959.130 kb on - strand, within SGL_RS15640 at 2959.267 kb on - strand, within SGL_RS15640 at 2959.361 kb on - strand at 2959.361 kb on - strand at 2959.564 kb on + strand at 2959.564 kb on + strand at 2959.643 kb on - strand at 2959.730 kb on + strand, within SGL_RS15645 at 2959.735 kb on - strand, within SGL_RS15645 at 2959.782 kb on + strand, within SGL_RS15645 at 2959.782 kb on + strand, within SGL_RS15645 at 2959.790 kb on - strand, within SGL_RS15645 at 2959.828 kb on - strand, within SGL_RS15645 at 2959.884 kb on - strand, within SGL_RS15645 at 2959.916 kb on - strand, within SGL_RS15645 at 2959.926 kb on + strand, within SGL_RS15645 at 2959.941 kb on - strand, within SGL_RS15645 at 2959.962 kb on - strand, within SGL_RS15645 at 2959.985 kb on + strand, within SGL_RS15645 at 2959.987 kb on - strand, within SGL_RS15645 at 2960.001 kb on + strand, within SGL_RS15645 at 2960.009 kb on + strand, within SGL_RS15645 at 2960.057 kb on - strand, within SGL_RS15645 at 2960.138 kb on + strand, within SGL_RS15645 at 2960.186 kb on - strand, within SGL_RS15645 at 2960.220 kb on + strand, within SGL_RS15645 at 2960.238 kb on + strand, within SGL_RS15645 at 2960.351 kb on - strand, within SGL_RS15645 at 2960.370 kb on + strand, within SGL_RS15645
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 2,957,119 + +0.2 2,957,169 + SGL_RS15630 0.18 -0.3 2,957,177 - SGL_RS15630 0.22 +0.9 2,957,178 - SGL_RS15630 0.22 +0.2 2,957,196 - SGL_RS15630 0.30 -0.4 2,957,199 + SGL_RS15630 0.31 +1.4 2,957,242 - SGL_RS15630 0.49 +0.8 2,957,242 - SGL_RS15630 0.49 +0.6 2,957,443 + +0.1 2,957,453 + -0.0 2,957,524 + SGL_RS15635 0.11 -0.0 2,957,526 + SGL_RS15635 0.11 -0.4 2,957,599 + SGL_RS15635 0.28 -0.4 2,957,599 + SGL_RS15635 0.28 -0.1 2,957,742 - SGL_RS15635 0.62 -0.6 2,957,793 - SGL_RS15635 0.74 +0.2 2,957,979 - -0.2 2,958,000 - +0.9 2,958,159 - +0.4 2,958,429 + SGL_RS15640 0.27 +0.4 2,958,437 - SGL_RS15640 0.28 -0.3 2,958,535 - SGL_RS15640 0.34 +0.3 2,958,627 - SGL_RS15640 0.41 -0.1 2,958,636 + SGL_RS15640 0.41 -0.5 2,958,652 - SGL_RS15640 0.42 -0.1 2,958,728 - SGL_RS15640 0.48 -0.1 2,958,733 - SGL_RS15640 0.48 +1.6 2,958,824 - SGL_RS15640 0.54 -0.5 2,958,844 - SGL_RS15640 0.56 +1.0 2,958,844 - SGL_RS15640 0.56 -0.1 2,958,850 - SGL_RS15640 0.56 +0.1 2,958,852 + SGL_RS15640 0.56 -0.3 2,958,953 - SGL_RS15640 0.63 -0.2 2,958,964 - SGL_RS15640 0.64 +0.3 2,958,996 - SGL_RS15640 0.66 -0.1 2,959,101 - SGL_RS15640 0.73 -0.3 2,959,130 - SGL_RS15640 0.75 -0.7 2,959,267 - SGL_RS15640 0.85 -0.1 2,959,361 - -0.3 2,959,361 - -0.6 2,959,564 + +0.9 2,959,564 + +0.5 2,959,643 - +0.6 2,959,730 + SGL_RS15645 0.16 +1.0 2,959,735 - SGL_RS15645 0.17 +0.2 2,959,782 + SGL_RS15645 0.21 +0.3 2,959,782 + SGL_RS15645 0.21 -0.4 2,959,790 - SGL_RS15645 0.21 -0.0 2,959,828 - SGL_RS15645 0.24 +0.6 2,959,884 - SGL_RS15645 0.29 +0.8 2,959,916 - SGL_RS15645 0.31 +0.7 2,959,926 + SGL_RS15645 0.32 +0.6 2,959,941 - SGL_RS15645 0.33 +0.4 2,959,962 - SGL_RS15645 0.35 +0.3 2,959,985 + SGL_RS15645 0.37 +1.1 2,959,987 - SGL_RS15645 0.37 +0.3 2,960,001 + SGL_RS15645 0.38 -0.1 2,960,009 + SGL_RS15645 0.39 -1.1 2,960,057 - SGL_RS15645 0.43 +0.6 2,960,138 + SGL_RS15645 0.50 +0.7 2,960,186 - SGL_RS15645 0.53 +0.0 2,960,220 + SGL_RS15645 0.56 +0.6 2,960,238 + SGL_RS15645 0.58 +0.7 2,960,351 - SGL_RS15645 0.67 +0.7 2,960,370 + SGL_RS15645 0.68 +1.0
Or see this region's nucleotide sequence