Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13460 and SGL_RS13465 are separated by 104 nucleotides SGL_RS13465 and SGL_RS19630 are separated by 471 nucleotides SGL_RS19630 and SGL_RS13470 are separated by 40 nucleotides SGL_RS13470 and SGL_RS13475 are separated by 198 nucleotides
SGL_RS13460: SGL_RS13460 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 2,486,607 to 2,487,923
_RS13460
SGL_RS13465: SGL_RS13465 - class II fumarate hydratase, at 2,488,028 to 2,489,428
_RS13465
SGL_RS19630: SGL_RS19630 - DUF4114 domain-containing protein, at 2,489,900 to 2,490,016
_RS19630
SGL_RS13470: SGL_RS13470 - tRNA-Gly, at 2,490,057 to 2,490,127
_RS13470
SGL_RS13475: SGL_RS13475 - fimbria/pilus outer membrane usher protein, at 2,490,326 to 2,493,004
_RS13475
Position (kb)
2488
2489
2490 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2487.906 kb on - strand at 2488.059 kb on + strand at 2488.090 kb on + strand at 2488.445 kb on - strand, within SGL_RS13465 at 2488.541 kb on - strand, within SGL_RS13465 at 2488.541 kb on - strand, within SGL_RS13465 at 2488.728 kb on + strand, within SGL_RS13465 at 2488.735 kb on + strand, within SGL_RS13465 at 2488.741 kb on - strand, within SGL_RS13465 at 2488.741 kb on - strand, within SGL_RS13465 at 2489.048 kb on - strand, within SGL_RS13465 at 2489.199 kb on + strand, within SGL_RS13465 at 2489.287 kb on - strand, within SGL_RS13465 at 2489.330 kb on + strand at 2489.890 kb on + strand at 2489.928 kb on + strand, within SGL_RS19630 at 2489.928 kb on + strand, within SGL_RS19630 at 2490.061 kb on + strand at 2490.188 kb on - strand at 2490.393 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 2,487,906 - -3.8 2,488,059 + -0.8 2,488,090 + -1.9 2,488,445 - SGL_RS13465 0.30 -5.2 2,488,541 - SGL_RS13465 0.37 -3.5 2,488,541 - SGL_RS13465 0.37 -3.1 2,488,728 + SGL_RS13465 0.50 -2.9 2,488,735 + SGL_RS13465 0.50 -3.2 2,488,741 - SGL_RS13465 0.51 -4.0 2,488,741 - SGL_RS13465 0.51 -3.0 2,489,048 - SGL_RS13465 0.73 -4.2 2,489,199 + SGL_RS13465 0.84 -3.5 2,489,287 - SGL_RS13465 0.90 -2.8 2,489,330 + -2.5 2,489,890 + -1.1 2,489,928 + SGL_RS19630 0.24 +0.4 2,489,928 + SGL_RS19630 0.24 +0.7 2,490,061 + -0.1 2,490,188 - -0.1 2,490,393 + -0.3
Or see this region's nucleotide sequence