Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12450 and SGL_RS12455 are separated by 64 nucleotides SGL_RS12455 and SGL_RS12460 are separated by 236 nucleotides SGL_RS12460 and SGL_RS12465 are separated by 160 nucleotides
SGL_RS12450: SGL_RS12450 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 2,248,578 to 2,250,176
_RS12450
SGL_RS12455: SGL_RS12455 - preprotein translocase subunit SecG, at 2,250,241 to 2,250,474
_RS12455
SGL_RS12460: SGL_RS12460 - matrixin family metalloprotease, at 2,250,711 to 2,251,496
_RS12460
SGL_RS12465: SGL_RS12465 - dihydrolipoamide acetyltransferase family protein, at 2,251,657 to 2,252,958
_RS12465
Position (kb)
2250
2251
2252 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2250.125 kb on + strand at 2250.145 kb on - strand at 2250.604 kb on - strand at 2250.781 kb on - strand at 2250.949 kb on - strand, within SGL_RS12460 at 2251.044 kb on - strand, within SGL_RS12460 at 2251.321 kb on + strand, within SGL_RS12460 at 2251.352 kb on + strand, within SGL_RS12460 at 2251.396 kb on - strand, within SGL_RS12460 at 2251.443 kb on - strand at 2251.464 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 2,250,125 + -0.2 2,250,145 - -0.5 2,250,604 - +0.2 2,250,781 - -4.0 2,250,949 - SGL_RS12460 0.30 -4.2 2,251,044 - SGL_RS12460 0.42 -4.0 2,251,321 + SGL_RS12460 0.78 -2.4 2,251,352 + SGL_RS12460 0.82 -5.4 2,251,396 - SGL_RS12460 0.87 -5.0 2,251,443 - -3.2 2,251,464 + -0.1
Or see this region's nucleotide sequence