Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11280 and SGL_RS11285 are separated by 102 nucleotides SGL_RS11285 and SGL_RS11290 are separated by 101 nucleotides SGL_RS11290 and SGL_RS11295 are separated by 245 nucleotides SGL_RS11295 and SGL_RS11300 are separated by 202 nucleotides
SGL_RS11280: SGL_RS11280 - serine/threonine-protein kinase, at 1,991,550 to 1,993,274
_RS11280
SGL_RS11285: SGL_RS11285 - response regulator transcription factor, at 1,993,377 to 1,994,045
_RS11285
SGL_RS11290: SGL_RS11290 - phosphoenolpyruvate carboxylase, at 1,994,147 to 1,997,251
_RS11290
SGL_RS11295: SGL_RS11295 - hypothetical protein, at 1,997,497 to 1,997,790
_RS11295
SGL_RS11300: SGL_RS11300 - NfeD family protein, at 1,997,993 to 1,998,451
_RS11300
Position (kb)
1994
1995
1996
1997
1998 Strain fitness (log2 ratio)
-1
0
1 at 1993.274 kb on + strand at 1993.365 kb on + strand at 1993.402 kb on + strand at 1993.464 kb on + strand, within SGL_RS11285 at 1994.046 kb on + strand at 1994.105 kb on - strand at 1994.115 kb on - strand at 1997.330 kb on - strand at 1997.906 kb on + strand at 1997.907 kb on - strand at 1998.029 kb on + strand at 1998.032 kb on - strand at 1998.039 kb on - strand, within SGL_RS11300
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 1,993,274 + -0.3 1,993,365 + +0.1 1,993,402 + +1.2 1,993,464 + SGL_RS11285 0.13 +1.3 1,994,046 + +0.3 1,994,105 - +1.2 1,994,115 - +0.6 1,997,330 - -0.4 1,997,906 + -0.1 1,997,907 - +0.3 1,998,029 + -0.8 1,998,032 - -0.5 1,998,039 - SGL_RS11300 0.10 +1.1
Or see this region's nucleotide sequence