Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11240 and SGL_RS11245 are separated by 34 nucleotides SGL_RS11245 and SGL_RS11250 are separated by 32 nucleotides SGL_RS11250 and SGL_RS11255 are separated by 49 nucleotides
SGL_RS11240: SGL_RS11240 - photosystem I reaction center subunit IV, at 1,982,049 to 1,982,273
_RS11240
SGL_RS11245: SGL_RS11245 - DNA-formamidopyrimidine glycosylase, at 1,982,308 to 1,983,171
_RS11245
SGL_RS11250: SGL_RS11250 - NUDIX domain-containing protein, at 1,983,204 to 1,983,932
_RS11250
SGL_RS11255: SGL_RS11255 - NAD+ synthase, at 1,983,982 to 1,985,658
_RS11255
Position (kb) 1983
1984 Strain fitness (log2 ratio) -1
0
1 at 1982.214 kb on - strand, within SGL_RS11240 at 1982.368 kb on - strand at 1982.553 kb on + strand, within SGL_RS11245 at 1982.597 kb on + strand, within SGL_RS11245 at 1982.617 kb on + strand, within SGL_RS11245 at 1982.625 kb on - strand, within SGL_RS11245 at 1982.649 kb on - strand, within SGL_RS11245 at 1982.731 kb on - strand, within SGL_RS11245 at 1982.930 kb on + strand, within SGL_RS11245 at 1982.956 kb on - strand, within SGL_RS11245 at 1982.998 kb on - strand, within SGL_RS11245 at 1983.062 kb on - strand, within SGL_RS11245 at 1983.089 kb on + strand at 1983.384 kb on + strand, within SGL_RS11250 at 1983.393 kb on - strand, within SGL_RS11250 at 1983.857 kb on - strand, within SGL_RS11250 at 1983.982 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 1,982,214 - SGL_RS11240 0.73 +0.7 1,982,368 - +0.5 1,982,553 + SGL_RS11245 0.28 -0.3 1,982,597 + SGL_RS11245 0.33 -0.5 1,982,617 + SGL_RS11245 0.36 -1.0 1,982,625 - SGL_RS11245 0.37 +0.6 1,982,649 - SGL_RS11245 0.39 -0.5 1,982,731 - SGL_RS11245 0.49 +0.3 1,982,930 + SGL_RS11245 0.72 -0.1 1,982,956 - SGL_RS11245 0.75 -0.1 1,982,998 - SGL_RS11245 0.80 +0.7 1,983,062 - SGL_RS11245 0.87 +1.0 1,983,089 + -0.1 1,983,384 + SGL_RS11250 0.25 -1.6 1,983,393 - SGL_RS11250 0.26 -0.8 1,983,857 - SGL_RS11250 0.90 -0.5 1,983,982 + +1.2
Or see this region's nucleotide sequence