Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09490 and SGL_RS19865 are separated by 69 nucleotides SGL_RS19865 and SGL_RS18860 overlap by 91 nucleotides SGL_RS18860 and SGL_RS09505 overlap by 32 nucleotides SGL_RS09505 and SGL_RS09510 are separated by 26 nucleotides
SGL_RS09490: SGL_RS09490 - CBS domain-containing protein, at 1,625,622 to 1,626,095
_RS09490
SGL_RS19865: SGL_RS19865 - IS630 transposase-related protein, at 1,626,165 to 1,626,524
_RS19865
SGL_RS18860: SGL_RS18860 - IS630 family transposase, at 1,626,434 to 1,627,012
_RS18860
SGL_RS09505: SGL_RS09505 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 1,626,981 to 1,628,018
_RS09505
SGL_RS09510: SGL_RS09510 - beta-3-deoxy-D-manno-oct-2-ulosonic acid transferase, at 1,628,045 to 1,629,607
_RS09510
Position (kb) 1626
1627
1628
1629 Strain fitness (log2 ratio) -1
0
1 at 1626.001 kb on - strand, within SGL_RS09490 at 1626.001 kb on - strand, within SGL_RS09490 at 1627.100 kb on + strand, within SGL_RS09505 at 1627.108 kb on - strand, within SGL_RS09505 at 1627.126 kb on - strand, within SGL_RS09505 at 1627.175 kb on + strand, within SGL_RS09505 at 1628.372 kb on - strand, within SGL_RS09510
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 1,626,001 - SGL_RS09490 0.80 +0.4 1,626,001 - SGL_RS09490 0.80 +0.2 1,627,100 + SGL_RS09505 0.11 +0.1 1,627,108 - SGL_RS09505 0.12 +0.2 1,627,126 - SGL_RS09505 0.14 -0.1 1,627,175 + SGL_RS09505 0.19 +0.2 1,628,372 - SGL_RS09510 0.21 +0.5
Or see this region's nucleotide sequence