Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09105 and SGL_RS09110 are separated by 213 nucleotides SGL_RS09110 and SGL_RS09115 are separated by 89 nucleotides SGL_RS09115 and SGL_RS09125 are separated by 542 nucleotides
SGL_RS09105: SGL_RS09105 - biosynthetic-type acetolactate synthase large subunit, at 1,548,873 to 1,550,738
_RS09105
SGL_RS09110: SGL_RS09110 - squalene--hopene cyclase, at 1,550,952 to 1,552,895
_RS09110
SGL_RS09115: SGL_RS09115 - tRNA-Pro, at 1,552,985 to 1,553,058
_RS09115
SGL_RS09125: SGL_RS09125 - IS701 family transposase, at 1,553,601 to 1,553,903
_RS09125
Position (kb)
1550
1551
1552
1553 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1550.741 kb on - strand at 1550.785 kb on - strand at 1551.070 kb on - strand at 1551.256 kb on - strand, within SGL_RS09110 at 1551.835 kb on + strand, within SGL_RS09110 at 1551.906 kb on + strand, within SGL_RS09110 at 1551.920 kb on - strand, within SGL_RS09110 at 1551.953 kb on + strand, within SGL_RS09110 at 1552.133 kb on - strand, within SGL_RS09110 at 1552.256 kb on - strand, within SGL_RS09110 at 1552.394 kb on - strand, within SGL_RS09110 at 1552.490 kb on - strand, within SGL_RS09110 at 1552.530 kb on + strand, within SGL_RS09110 at 1552.635 kb on - strand, within SGL_RS09110 at 1552.771 kb on + strand at 1552.918 kb on - strand at 1553.099 kb on - strand at 1553.164 kb on + strand at 1553.164 kb on + strand at 1553.219 kb on - strand at 1553.221 kb on + strand at 1553.239 kb on + strand at 1553.872 kb on + strand, within SGL_RS09125
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 1,550,741 - -0.3 1,550,785 - -0.6 1,551,070 - -2.3 1,551,256 - SGL_RS09110 0.16 -0.6 1,551,835 + SGL_RS09110 0.45 -3.2 1,551,906 + SGL_RS09110 0.49 -1.9 1,551,920 - SGL_RS09110 0.50 -2.1 1,551,953 + SGL_RS09110 0.51 -3.5 1,552,133 - SGL_RS09110 0.61 -2.1 1,552,256 - SGL_RS09110 0.67 -0.9 1,552,394 - SGL_RS09110 0.74 -0.3 1,552,490 - SGL_RS09110 0.79 -2.5 1,552,530 + SGL_RS09110 0.81 -2.1 1,552,635 - SGL_RS09110 0.87 -2.0 1,552,771 + -1.1 1,552,918 - -0.6 1,553,099 - -0.5 1,553,164 + +0.7 1,553,164 + -0.1 1,553,219 - +0.6 1,553,221 + +0.5 1,553,239 + +0.4 1,553,872 + SGL_RS09125 0.89 +0.1
Or see this region's nucleotide sequence