Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08640 and SGL_RS18805 are separated by 32 nucleotides SGL_RS18805 and SGL_RS08650 are separated by 38 nucleotides SGL_RS08650 and SGL_RS08655 are separated by 257 nucleotides
SGL_RS08640: SGL_RS08640 - NAD(P)/FAD-dependent oxidoreductase, at 1,444,115 to 1,445,392
_RS08640
SGL_RS18805: SGL_RS18805 - type II toxin-antitoxin system HicA family toxin, at 1,445,425 to 1,445,835
_RS18805
SGL_RS08650: SGL_RS08650 - S-layer homology domain-containing protein, at 1,445,874 to 1,446,839
_RS08650
SGL_RS08655: SGL_RS08655 - cyanophycinase, at 1,447,097 to 1,447,912
_RS08655
Position (kb)
1445
1446
1447 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1444.901 kb on - strand, within SGL_RS08640 at 1444.943 kb on + strand, within SGL_RS08640 at 1444.946 kb on - strand, within SGL_RS08640 at 1444.951 kb on - strand, within SGL_RS08640 at 1444.951 kb on - strand, within SGL_RS08640 at 1445.305 kb on - strand at 1445.381 kb on + strand at 1445.395 kb on - strand at 1445.582 kb on + strand, within SGL_RS18805 at 1445.612 kb on + strand, within SGL_RS18805 at 1446.303 kb on + strand, within SGL_RS08650 at 1446.347 kb on - strand, within SGL_RS08650 at 1446.347 kb on - strand, within SGL_RS08650 at 1446.397 kb on + strand, within SGL_RS08650 at 1446.699 kb on - strand, within SGL_RS08650 at 1446.712 kb on - strand, within SGL_RS08650 at 1446.795 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 1,444,901 - SGL_RS08640 0.62 -1.4 1,444,943 + SGL_RS08640 0.65 -1.4 1,444,946 - SGL_RS08640 0.65 -0.8 1,444,951 - SGL_RS08640 0.65 -1.2 1,444,951 - SGL_RS08640 0.65 -0.9 1,445,305 - -1.1 1,445,381 + -0.3 1,445,395 - -0.6 1,445,582 + SGL_RS18805 0.38 -0.7 1,445,612 + SGL_RS18805 0.45 -1.0 1,446,303 + SGL_RS08650 0.44 -3.9 1,446,347 - SGL_RS08650 0.49 -2.3 1,446,347 - SGL_RS08650 0.49 -3.8 1,446,397 + SGL_RS08650 0.54 -4.4 1,446,699 - SGL_RS08650 0.85 -2.5 1,446,712 - SGL_RS08650 0.87 -3.3 1,446,795 - +0.1
Or see this region's nucleotide sequence