Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06480 and SGL_RS06485 are separated by 182 nucleotides SGL_RS06485 and SGL_RS06490 are separated by 23 nucleotides SGL_RS06490 and SGL_RS06495 are separated by 74 nucleotides SGL_RS06495 and SGL_RS06500 are separated by 114 nucleotides
SGL_RS06480: SGL_RS06480 - sigma-70 family RNA polymerase sigma factor, at 983,804 to 984,475
_RS06480
SGL_RS06485: SGL_RS06485 - anti-sigma factor, at 984,658 to 985,200
_RS06485
SGL_RS06490: SGL_RS06490 - permease, at 985,224 to 986,222
_RS06490
SGL_RS06495: SGL_RS06495 - tRNA-Leu, at 986,297 to 986,377
_RS06495
SGL_RS06500: SGL_RS06500 - glycosyltransferase family 4 protein, at 986,492 to 987,733
_RS06500
Position (kb) 985
986
987 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 984.365 kb on - strand, within SGL_RS06480 at 984.430 kb on - strand at 984.552 kb on + strand at 984.588 kb on + strand at 985.000 kb on + strand, within SGL_RS06485 at 985.387 kb on + strand, within SGL_RS06490 at 985.573 kb on + strand, within SGL_RS06490 at 985.750 kb on + strand, within SGL_RS06490 at 986.033 kb on - strand, within SGL_RS06490 at 986.110 kb on + strand, within SGL_RS06490 at 986.490 kb on + strand at 986.525 kb on + strand at 986.558 kb on + strand at 986.730 kb on - strand, within SGL_RS06500 at 986.787 kb on - strand, within SGL_RS06500 at 986.787 kb on - strand, within SGL_RS06500
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 984,365 - SGL_RS06480 0.83 -1.3 984,430 - -0.9 984,552 + +1.2 984,588 + +2.2 985,000 + SGL_RS06485 0.63 -3.6 985,387 + SGL_RS06490 0.16 -2.8 985,573 + SGL_RS06490 0.35 -3.7 985,750 + SGL_RS06490 0.53 -1.1 986,033 - SGL_RS06490 0.81 -3.1 986,110 + SGL_RS06490 0.89 -0.7 986,490 + -1.9 986,525 + -0.8 986,558 + +0.3 986,730 - SGL_RS06500 0.19 +0.8 986,787 - SGL_RS06500 0.24 -0.1 986,787 - SGL_RS06500 0.24 +0.2
Or see this region's nucleotide sequence