Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04415 and SGL_RS04420 overlap by 4 nucleotides SGL_RS04420 and SGL_RS04425 are separated by 292 nucleotides SGL_RS04425 and SGL_RS04430 are separated by 203 nucleotides
SGL_RS04415: SGL_RS04415 - prohibitin family protein, at 548,236 to 549,132
_RS04415
SGL_RS04420: SGL_RS04420 - gamma carbonic anhydrase family protein, at 549,129 to 549,701
_RS04420
SGL_RS04425: SGL_RS04425 - hypothetical protein, at 549,994 to 550,329
_RS04425
SGL_RS04430: SGL_RS04430 - CocE/NonD family hydrolase, at 550,533 to 552,170
_RS04430
Position (kb) 549
550 Strain fitness (log2 ratio) -1
0
1 at 548.368 kb on + strand, within SGL_RS04415 at 548.462 kb on - strand, within SGL_RS04415 at 548.464 kb on + strand, within SGL_RS04415 at 548.466 kb on + strand, within SGL_RS04415 at 548.466 kb on + strand, within SGL_RS04415 at 548.466 kb on + strand, within SGL_RS04415 at 548.479 kb on - strand, within SGL_RS04415 at 548.616 kb on + strand, within SGL_RS04415 at 548.750 kb on + strand, within SGL_RS04415 at 549.153 kb on + strand at 549.204 kb on - strand, within SGL_RS04420 at 549.204 kb on - strand, within SGL_RS04420 at 549.214 kb on - strand, within SGL_RS04420 at 549.214 kb on - strand, within SGL_RS04420 at 549.277 kb on - strand, within SGL_RS04420 at 549.355 kb on + strand, within SGL_RS04420 at 549.368 kb on - strand, within SGL_RS04420 at 549.387 kb on - strand, within SGL_RS04420 at 549.513 kb on + strand, within SGL_RS04420 at 549.515 kb on + strand, within SGL_RS04420 at 549.642 kb on + strand, within SGL_RS04420 at 549.682 kb on + strand at 549.961 kb on + strand at 550.095 kb on - strand, within SGL_RS04425 at 550.100 kb on + strand, within SGL_RS04425 at 550.394 kb on - strand at 550.451 kb on - strand at 550.471 kb on + strand at 550.506 kb on - strand at 550.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 548,368 + SGL_RS04415 0.15 +0.3 548,462 - SGL_RS04415 0.25 +0.3 548,464 + SGL_RS04415 0.25 -0.3 548,466 + SGL_RS04415 0.26 +0.0 548,466 + SGL_RS04415 0.26 +0.1 548,466 + SGL_RS04415 0.26 +0.5 548,479 - SGL_RS04415 0.27 +0.2 548,616 + SGL_RS04415 0.42 +0.4 548,750 + SGL_RS04415 0.57 -0.9 549,153 + +0.0 549,204 - SGL_RS04420 0.13 +0.2 549,204 - SGL_RS04420 0.13 +0.2 549,214 - SGL_RS04420 0.15 +0.6 549,214 - SGL_RS04420 0.15 -0.2 549,277 - SGL_RS04420 0.26 -0.2 549,355 + SGL_RS04420 0.39 -0.2 549,368 - SGL_RS04420 0.42 -0.3 549,387 - SGL_RS04420 0.45 +0.6 549,513 + SGL_RS04420 0.67 +0.1 549,515 + SGL_RS04420 0.67 +0.4 549,642 + SGL_RS04420 0.90 -0.2 549,682 + +0.0 549,961 + +1.3 550,095 - SGL_RS04425 0.30 +0.0 550,100 + SGL_RS04425 0.32 -0.5 550,394 - -0.2 550,451 - -0.7 550,471 + +1.0 550,506 - -0.4 550,688 - +0.4
Or see this region's nucleotide sequence