Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04235 and SGL_RS04240 are separated by 75 nucleotides SGL_RS04240 and SGL_RS04245 are separated by 98 nucleotides SGL_RS04245 and SGL_RS04250 are separated by 195 nucleotides
SGL_RS04235: SGL_RS04235 - pentapeptide repeat-containing protein, at 501,178 to 501,966
_RS04235
SGL_RS04240: SGL_RS04240 - 50S ribosomal protein L32, at 502,042 to 502,215
_RS04240
SGL_RS04245: SGL_RS04245 - dolichyl-phosphate-mannose--protein mannosyltransferase, at 502,314 to 503,747
_RS04245
SGL_RS04250: SGL_RS04250 - enoyl-ACP reductase FabI, at 503,943 to 504,719
_RS04250
Position (kb) 502
503
504 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 501.660 kb on + strand, within SGL_RS04235 at 501.674 kb on + strand, within SGL_RS04235 at 501.676 kb on - strand, within SGL_RS04235 at 501.910 kb on - strand at 501.920 kb on - strand at 501.932 kb on - strand at 501.966 kb on - strand at 502.266 kb on - strand at 502.529 kb on + strand, within SGL_RS04245 at 502.591 kb on - strand, within SGL_RS04245 at 502.673 kb on + strand, within SGL_RS04245 at 502.764 kb on - strand, within SGL_RS04245 at 502.863 kb on + strand, within SGL_RS04245 at 502.866 kb on - strand, within SGL_RS04245 at 502.871 kb on - strand, within SGL_RS04245 at 502.951 kb on - strand, within SGL_RS04245 at 503.255 kb on - strand, within SGL_RS04245 at 503.269 kb on + strand, within SGL_RS04245 at 503.373 kb on + strand, within SGL_RS04245 at 503.440 kb on - strand, within SGL_RS04245 at 503.445 kb on + strand, within SGL_RS04245 at 503.555 kb on - strand, within SGL_RS04245 at 503.602 kb on + strand, within SGL_RS04245 at 503.610 kb on - strand at 503.637 kb on + strand at 503.716 kb on - strand at 503.735 kb on - strand at 504.439 kb on - strand, within SGL_RS04250 at 504.530 kb on - strand, within SGL_RS04250
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 501,660 + SGL_RS04235 0.61 -0.2 501,674 + SGL_RS04235 0.63 +0.2 501,676 - SGL_RS04235 0.63 +0.3 501,910 - +0.3 501,920 - -0.2 501,932 - -0.3 501,966 - +0.3 502,266 - -0.4 502,529 + SGL_RS04245 0.15 -1.3 502,591 - SGL_RS04245 0.19 -2.5 502,673 + SGL_RS04245 0.25 -1.9 502,764 - SGL_RS04245 0.31 -1.4 502,863 + SGL_RS04245 0.38 -0.9 502,866 - SGL_RS04245 0.38 -1.5 502,871 - SGL_RS04245 0.39 -2.9 502,951 - SGL_RS04245 0.44 -2.2 503,255 - SGL_RS04245 0.66 -2.5 503,269 + SGL_RS04245 0.67 -3.2 503,373 + SGL_RS04245 0.74 -1.3 503,440 - SGL_RS04245 0.79 -3.2 503,445 + SGL_RS04245 0.79 -2.3 503,555 - SGL_RS04245 0.87 -2.6 503,602 + SGL_RS04245 0.90 -2.7 503,610 - -1.7 503,637 + -3.5 503,716 - -2.4 503,735 - -3.3 504,439 - SGL_RS04250 0.64 -0.3 504,530 - SGL_RS04250 0.76 -0.9
Or see this region's nucleotide sequence