Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03400 and SGL_RS03405 are separated by 51 nucleotides SGL_RS03405 and SGL_RS03410 are separated by 24 nucleotides SGL_RS03410 and SGL_RS03415 are separated by 40 nucleotides SGL_RS03415 and SGL_RS03420 are separated by 33 nucleotides
SGL_RS03400: SGL_RS03400 - pentapeptide repeat-containing protein, at 327,100 to 327,552
_RS03400
SGL_RS03405: SGL_RS03405 - DUF1622 domain-containing protein, at 327,604 to 327,981
_RS03405
SGL_RS03410: SGL_RS03410 - LL-diaminopimelate aminotransferase, at 328,006 to 329,181
_RS03410
SGL_RS03415: SGL_RS03415 - SH3 domain-containing protein, at 329,222 to 329,521
_RS03415
SGL_RS03420: SGL_RS03420 - UbiD family decarboxylase, at 329,555 to 331,060
_RS03420
Position (kb) 328
329
330 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 327.278 kb on + strand, within SGL_RS03400 at 327.391 kb on - strand, within SGL_RS03400 at 327.458 kb on - strand, within SGL_RS03400 at 327.463 kb on + strand, within SGL_RS03400 at 327.792 kb on - strand, within SGL_RS03405 at 327.983 kb on + strand at 328.376 kb on - strand, within SGL_RS03410 at 328.462 kb on - strand, within SGL_RS03410 at 328.598 kb on + strand, within SGL_RS03410 at 328.603 kb on + strand, within SGL_RS03410 at 328.690 kb on + strand, within SGL_RS03410 at 328.698 kb on - strand, within SGL_RS03410 at 328.712 kb on + strand, within SGL_RS03410 at 328.720 kb on - strand, within SGL_RS03410 at 328.801 kb on + strand, within SGL_RS03410 at 328.822 kb on - strand, within SGL_RS03410 at 329.019 kb on - strand, within SGL_RS03410 at 329.053 kb on + strand, within SGL_RS03410 at 329.097 kb on - strand at 329.100 kb on + strand at 329.627 kb on + strand at 329.651 kb on + strand at 329.663 kb on + strand at 329.674 kb on - strand at 329.868 kb on - strand, within SGL_RS03420 at 330.016 kb on - strand, within SGL_RS03420 at 330.068 kb on + strand, within SGL_RS03420
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 327,278 + SGL_RS03400 0.39 +0.3 327,391 - SGL_RS03400 0.64 -0.2 327,458 - SGL_RS03400 0.79 +0.2 327,463 + SGL_RS03400 0.80 +0.5 327,792 - SGL_RS03405 0.50 -0.4 327,983 + +0.9 328,376 - SGL_RS03410 0.31 +0.1 328,462 - SGL_RS03410 0.39 -1.1 328,598 + SGL_RS03410 0.50 +0.0 328,603 + SGL_RS03410 0.51 -0.7 328,690 + SGL_RS03410 0.58 -0.4 328,698 - SGL_RS03410 0.59 -0.5 328,712 + SGL_RS03410 0.60 -0.4 328,720 - SGL_RS03410 0.61 -1.4 328,801 + SGL_RS03410 0.68 -1.1 328,822 - SGL_RS03410 0.69 -0.9 329,019 - SGL_RS03410 0.86 -1.1 329,053 + SGL_RS03410 0.89 -0.3 329,097 - +0.1 329,100 + -0.0 329,627 + -1.8 329,651 + -3.3 329,663 + -1.0 329,674 - -2.7 329,868 - SGL_RS03420 0.21 -2.0 330,016 - SGL_RS03420 0.31 -1.6 330,068 + SGL_RS03420 0.34 -1.2
Or see this region's nucleotide sequence