Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03195 and SGL_RS19760 are separated by 66 nucleotides SGL_RS19760 and SGL_RS19765 overlap by 32 nucleotides SGL_RS19765 and SGL_RS03210 are separated by 238 nucleotides SGL_RS03210 and SGL_RS03215 are separated by 20 nucleotides SGL_RS03215 and SGL_RS03220 are separated by 63 nucleotides
SGL_RS03195: SGL_RS03195 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 281,507 to 282,223
_RS03195
SGL_RS19760: SGL_RS19760 - CopG family antitoxin, at 282,290 to 282,541
_RS19760
SGL_RS19765: SGL_RS19765 - BrnT family toxin, at 282,510 to 282,821
_RS19765
SGL_RS03210: SGL_RS03210 - NADP-dependent isocitrate dehydrogenase, at 283,060 to 284,487
_RS03210
SGL_RS03215: SGL_RS03215 - Uma2 family endonuclease, at 284,508 to 285,080
_RS03215
SGL_RS03220: SGL_RS03220 - Rpn family recombination-promoting nuclease/putative transposase, at 285,144 to 285,938
_RS03220
Position (kb) 283
284
285 Strain fitness (log2 ratio) -2
-1
0
1 at 282.070 kb on + strand, within SGL_RS03195 at 282.174 kb on - strand at 282.563 kb on + strand, within SGL_RS19765 at 282.573 kb on - strand, within SGL_RS19765 at 282.664 kb on - strand, within SGL_RS19765 at 282.879 kb on - strand at 283.391 kb on + strand, within SGL_RS03210 at 283.451 kb on + strand, within SGL_RS03210 at 283.627 kb on - strand, within SGL_RS03210 at 283.783 kb on - strand, within SGL_RS03210 at 284.238 kb on + strand, within SGL_RS03210 at 284.296 kb on - strand, within SGL_RS03210 at 284.519 kb on + strand at 284.527 kb on - strand at 284.612 kb on + strand, within SGL_RS03215 at 284.758 kb on - strand, within SGL_RS03215 at 284.761 kb on + strand, within SGL_RS03215 at 284.787 kb on - strand, within SGL_RS03215 at 284.905 kb on - strand, within SGL_RS03215 at 284.918 kb on - strand, within SGL_RS03215 at 285.033 kb on + strand at 285.133 kb on - strand at 285.360 kb on + strand, within SGL_RS03220 at 285.370 kb on + strand, within SGL_RS03220 at 285.380 kb on + strand, within SGL_RS03220 at 285.380 kb on + strand, within SGL_RS03220
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 282,070 + SGL_RS03195 0.79 +1.0 282,174 - -0.4 282,563 + SGL_RS19765 0.17 -0.1 282,573 - SGL_RS19765 0.20 +0.2 282,664 - SGL_RS19765 0.49 +0.5 282,879 - +0.2 283,391 + SGL_RS03210 0.23 -0.2 283,451 + SGL_RS03210 0.27 -1.5 283,627 - SGL_RS03210 0.40 -1.8 283,783 - SGL_RS03210 0.51 +0.8 284,238 + SGL_RS03210 0.82 +0.8 284,296 - SGL_RS03210 0.87 -0.2 284,519 + +0.1 284,527 - +0.3 284,612 + SGL_RS03215 0.18 +0.2 284,758 - SGL_RS03215 0.44 +1.1 284,761 + SGL_RS03215 0.44 -0.1 284,787 - SGL_RS03215 0.49 +0.1 284,905 - SGL_RS03215 0.69 +0.1 284,918 - SGL_RS03215 0.72 +0.4 285,033 + +0.3 285,133 - +0.1 285,360 + SGL_RS03220 0.27 -0.4 285,370 + SGL_RS03220 0.28 -0.2 285,380 + SGL_RS03220 0.30 +0.3 285,380 + SGL_RS03220 0.30 +0.6
Or see this region's nucleotide sequence