Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02975 and SGL_RS02980 are separated by 100 nucleotides SGL_RS02980 and SGL_RS02985 are separated by 33 nucleotides SGL_RS02985 and SGL_RS02990 are separated by 351 nucleotides
SGL_RS02975: SGL_RS02975 - transglutaminase domain-containing protein, at 238,804 to 240,198
_RS02975
SGL_RS02980: SGL_RS02980 - peptide-methionine (R)-S-oxide reductase MsrB, at 240,299 to 240,829
_RS02980
SGL_RS02985: SGL_RS02985 - HhoA/HhoB/HtrA family serine endopeptidase, at 240,863 to 242,047
_RS02985
SGL_RS02990: SGL_RS02990 - two-component system response regulator Rre1, at 242,399 to 243,094
_RS02990
Position (kb) 240
241
242
243 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 239.942 kb on - strand, within SGL_RS02975 at 240.071 kb on + strand at 240.080 kb on - strand at 240.133 kb on + strand at 240.195 kb on - strand at 240.314 kb on + strand at 240.322 kb on - strand at 240.391 kb on + strand, within SGL_RS02980 at 240.480 kb on + strand, within SGL_RS02980 at 240.632 kb on - strand, within SGL_RS02980 at 240.633 kb on - strand, within SGL_RS02980 at 240.640 kb on - strand, within SGL_RS02980 at 240.677 kb on - strand, within SGL_RS02980 at 240.688 kb on - strand, within SGL_RS02980 at 240.726 kb on + strand, within SGL_RS02980 at 240.850 kb on + strand at 240.929 kb on - strand at 240.929 kb on - strand at 240.985 kb on - strand, within SGL_RS02985 at 240.990 kb on - strand, within SGL_RS02985 at 241.040 kb on - strand, within SGL_RS02985 at 241.235 kb on + strand, within SGL_RS02985 at 241.301 kb on + strand, within SGL_RS02985 at 241.540 kb on - strand, within SGL_RS02985 at 241.545 kb on + strand, within SGL_RS02985 at 241.705 kb on + strand, within SGL_RS02985 at 241.707 kb on + strand, within SGL_RS02985 at 241.715 kb on - strand, within SGL_RS02985 at 241.819 kb on + strand, within SGL_RS02985 at 241.819 kb on - strand, within SGL_RS02985 at 241.819 kb on - strand, within SGL_RS02985 at 241.867 kb on - strand, within SGL_RS02985 at 241.924 kb on - strand, within SGL_RS02985 at 241.976 kb on + strand at 242.165 kb on - strand at 242.232 kb on - strand at 242.234 kb on + strand at 242.250 kb on + strand at 242.294 kb on + strand at 242.316 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 239,942 - SGL_RS02975 0.82 -1.5 240,071 + -2.4 240,080 - -2.2 240,133 + -3.2 240,195 - -1.2 240,314 + -2.0 240,322 - -1.9 240,391 + SGL_RS02980 0.17 -3.0 240,480 + SGL_RS02980 0.34 -1.7 240,632 - SGL_RS02980 0.63 -1.8 240,633 - SGL_RS02980 0.63 -2.6 240,640 - SGL_RS02980 0.64 -1.9 240,677 - SGL_RS02980 0.71 -2.4 240,688 - SGL_RS02980 0.73 -2.1 240,726 + SGL_RS02980 0.80 -1.9 240,850 + -1.5 240,929 - -3.0 240,929 - -0.9 240,985 - SGL_RS02985 0.10 -2.9 240,990 - SGL_RS02985 0.11 -4.2 241,040 - SGL_RS02985 0.15 -3.1 241,235 + SGL_RS02985 0.31 -2.6 241,301 + SGL_RS02985 0.37 -3.5 241,540 - SGL_RS02985 0.57 -3.8 241,545 + SGL_RS02985 0.58 -3.1 241,705 + SGL_RS02985 0.71 -2.9 241,707 + SGL_RS02985 0.71 -3.9 241,715 - SGL_RS02985 0.72 -1.8 241,819 + SGL_RS02985 0.81 -2.8 241,819 - SGL_RS02985 0.81 -2.4 241,819 - SGL_RS02985 0.81 -2.4 241,867 - SGL_RS02985 0.85 -2.8 241,924 - SGL_RS02985 0.90 -2.9 241,976 + -1.9 242,165 - -0.9 242,232 - +0.4 242,234 + -0.4 242,250 + -0.2 242,294 + -1.4 242,316 - -1.1
Or see this region's nucleotide sequence