Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02235 and SGL_RS02240 are separated by 268 nucleotides SGL_RS02240 and SGL_RS02245 are separated by 78 nucleotides SGL_RS02245 and SGL_RS02250 are separated by 51 nucleotides
SGL_RS02235: SGL_RS02235 - efflux RND transporter periplasmic adaptor subunit, at 82,291 to 83,853
_RS02235
SGL_RS02240: SGL_RS02240 - guanylate kinase, at 84,122 to 84,697
_RS02240
SGL_RS02245: SGL_RS02245 - histidine phosphatase family protein, at 84,776 to 86,107
_RS02245
SGL_RS02250: SGL_RS02250 - glycosyltransferase, at 86,159 to 87,418
_RS02250
Position (kb) 84
85
86
87 Strain fitness (log2 ratio) -1
0
1 at 83.868 kb on - strand at 84.007 kb on + strand at 84.035 kb on - strand at 84.679 kb on + strand at 84.724 kb on + strand at 84.726 kb on + strand at 84.726 kb on + strand at 84.728 kb on - strand at 86.099 kb on + strand at 86.107 kb on - strand at 86.200 kb on + strand at 86.221 kb on + strand at 86.229 kb on - strand at 86.259 kb on + strand at 86.404 kb on - strand, within SGL_RS02250 at 86.609 kb on - strand, within SGL_RS02250 at 86.609 kb on - strand, within SGL_RS02250 at 86.990 kb on - strand, within SGL_RS02250 at 87.102 kb on + strand, within SGL_RS02250
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 83,868 - +0.3 84,007 + -0.1 84,035 - -0.2 84,679 + -0.6 84,724 + -0.4 84,726 + -0.1 84,726 + +0.5 84,728 - +0.0 86,099 + +0.0 86,107 - +0.5 86,200 + +1.0 86,221 + -0.0 86,229 - +0.1 86,259 + +0.5 86,404 - SGL_RS02250 0.19 +0.2 86,609 - SGL_RS02250 0.36 -0.1 86,609 - SGL_RS02250 0.36 -0.2 86,990 - SGL_RS02250 0.66 +0.5 87,102 + SGL_RS02250 0.75 +0.5
Or see this region's nucleotide sequence