Experiment: Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18325 and SGL_RS18330 are separated by 143 nucleotides SGL_RS18330 and SGL_RS18335 are separated by 45 nucleotides SGL_RS18335 and SGL_RS18340 are separated by 17 nucleotides SGL_RS18340 and SGL_RS18345 are separated by 280 nucleotides
SGL_RS18325: SGL_RS18325 - NAD(P)/FAD-dependent oxidoreductase, at 3,559,199 to 3,560,179
_RS18325
SGL_RS18330: SGL_RS18330 - hypothetical protein, at 3,560,323 to 3,560,709
_RS18330
SGL_RS18335: SGL_RS18335 - hypothetical protein, at 3,560,755 to 3,561,144
_RS18335
SGL_RS18340: SGL_RS18340 - hypothetical protein, at 3,561,162 to 3,561,665
_RS18340
SGL_RS18345: SGL_RS18345 - trans-splicing intein-formed DNA polymerase III subunit alpha N-terminal partner DnaE-N, at 3,561,946 to 3,564,639
_RS18345
Position (kb) 3560
3561
3562 Strain fitness (log2 ratio) -1
0
1 at 3559.769 kb on + strand, within SGL_RS18325 at 3559.791 kb on - strand, within SGL_RS18325 at 3559.845 kb on + strand, within SGL_RS18325 at 3559.913 kb on + strand, within SGL_RS18325 at 3559.986 kb on - strand, within SGL_RS18325 at 3560.099 kb on - strand at 3560.259 kb on - strand at 3560.275 kb on + strand at 3560.465 kb on + strand, within SGL_RS18330 at 3560.500 kb on + strand, within SGL_RS18330 at 3560.542 kb on - strand, within SGL_RS18330 at 3560.623 kb on + strand, within SGL_RS18330 at 3560.623 kb on + strand, within SGL_RS18330 at 3560.631 kb on - strand, within SGL_RS18330 at 3560.667 kb on + strand, within SGL_RS18330 at 3560.744 kb on + strand at 3560.800 kb on - strand, within SGL_RS18335 at 3560.957 kb on + strand, within SGL_RS18335 at 3561.372 kb on + strand, within SGL_RS18340
Per-strain Table
Position Strand Gene LocusTag Fraction Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library) remove 3,559,769 + SGL_RS18325 0.58 -0.2 3,559,791 - SGL_RS18325 0.60 +0.3 3,559,845 + SGL_RS18325 0.66 -0.1 3,559,913 + SGL_RS18325 0.73 -0.1 3,559,986 - SGL_RS18325 0.80 +0.2 3,560,099 - +0.0 3,560,259 - +0.0 3,560,275 + +0.0 3,560,465 + SGL_RS18330 0.37 +0.9 3,560,500 + SGL_RS18330 0.46 +0.4 3,560,542 - SGL_RS18330 0.57 +0.1 3,560,623 + SGL_RS18330 0.78 +0.2 3,560,623 + SGL_RS18330 0.78 -0.1 3,560,631 - SGL_RS18330 0.80 -0.3 3,560,667 + SGL_RS18330 0.89 -0.2 3,560,744 + +0.2 3,560,800 - SGL_RS18335 0.12 -0.7 3,560,957 + SGL_RS18335 0.52 -0.8 3,561,372 + SGL_RS18340 0.42 +0.3
Or see this region's nucleotide sequence