Experiment: Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15295 and SGL_RS15300 are separated by 100 nucleotides SGL_RS15300 and SGL_RS15305 are separated by 80 nucleotides SGL_RS15305 and SGL_RS15310 are separated by 182 nucleotides
SGL_RS15295: SGL_RS15295 - GspE/PulE family protein, at 2,881,378 to 2,883,819
_RS15295
SGL_RS15300: SGL_RS15300 - hypothetical protein, at 2,883,920 to 2,884,276
_RS15300
SGL_RS15305: SGL_RS15305 - ribonuclease HI, at 2,884,357 to 2,884,839
_RS15305
SGL_RS15310: SGL_RS15310 - response regulator transcription factor, at 2,885,022 to 2,885,810
_RS15310
Position (kb)
2884
2885 Strain fitness (log2 ratio)
-1
0
1 at 2883.584 kb on + strand at 2883.637 kb on - strand at 2883.641 kb on + strand at 2883.720 kb on + strand at 2883.810 kb on - strand at 2884.095 kb on - strand, within SGL_RS15300 at 2884.136 kb on - strand, within SGL_RS15300 at 2884.313 kb on - strand at 2884.313 kb on - strand at 2884.333 kb on + strand at 2884.334 kb on + strand at 2884.341 kb on - strand at 2884.383 kb on + strand at 2884.401 kb on - strand at 2884.420 kb on - strand, within SGL_RS15305 at 2884.459 kb on - strand, within SGL_RS15305 at 2884.701 kb on + strand, within SGL_RS15305 at 2884.731 kb on + strand, within SGL_RS15305 at 2885.075 kb on + strand at 2885.075 kb on + strand at 2885.081 kb on - strand at 2885.083 kb on - strand at 2885.226 kb on - strand, within SGL_RS15310 at 2885.261 kb on - strand, within SGL_RS15310 at 2885.263 kb on + strand, within SGL_RS15310 at 2885.263 kb on + strand, within SGL_RS15310 at 2885.268 kb on + strand, within SGL_RS15310 at 2885.270 kb on + strand, within SGL_RS15310 at 2885.285 kb on - strand, within SGL_RS15310 at 2885.378 kb on - strand, within SGL_RS15310 at 2885.428 kb on + strand, within SGL_RS15310 at 2885.431 kb on - strand, within SGL_RS15310 at 2885.435 kb on + strand, within SGL_RS15310 at 2885.445 kb on - strand, within SGL_RS15310 at 2885.575 kb on + strand, within SGL_RS15310 at 2885.787 kb on - strand at 2885.833 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library) remove 2,883,584 + -0.1 2,883,637 - +0.3 2,883,641 + +0.0 2,883,720 + +0.4 2,883,810 - +0.1 2,884,095 - SGL_RS15300 0.49 -0.1 2,884,136 - SGL_RS15300 0.61 -0.5 2,884,313 - +0.1 2,884,313 - -0.6 2,884,333 + +0.2 2,884,334 + +0.1 2,884,341 - -0.0 2,884,383 + -0.3 2,884,401 - -1.1 2,884,420 - SGL_RS15305 0.13 -0.6 2,884,459 - SGL_RS15305 0.21 -0.1 2,884,701 + SGL_RS15305 0.71 -1.0 2,884,731 + SGL_RS15305 0.77 -0.6 2,885,075 + -0.2 2,885,075 + +0.1 2,885,081 - -0.1 2,885,083 - -0.1 2,885,226 - SGL_RS15310 0.26 +0.2 2,885,261 - SGL_RS15310 0.30 +0.1 2,885,263 + SGL_RS15310 0.31 +0.5 2,885,263 + SGL_RS15310 0.31 +0.1 2,885,268 + SGL_RS15310 0.31 -0.1 2,885,270 + SGL_RS15310 0.31 -0.2 2,885,285 - SGL_RS15310 0.33 +0.0 2,885,378 - SGL_RS15310 0.45 +0.2 2,885,428 + SGL_RS15310 0.51 +0.4 2,885,431 - SGL_RS15310 0.52 +0.1 2,885,435 + SGL_RS15310 0.52 +0.1 2,885,445 - SGL_RS15310 0.54 +0.3 2,885,575 + SGL_RS15310 0.70 -0.0 2,885,787 - +0.9 2,885,833 + -0.1
Or see this region's nucleotide sequence