Experiment: Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12375 and SGL_RS19925 are separated by 78 nucleotides SGL_RS19925 and SGL_RS18970 overlap by 91 nucleotides SGL_RS18970 and SGL_RS12390 are separated by 10 nucleotides SGL_RS12390 and SGL_RS12395 are separated by 79 nucleotides
SGL_RS12375: SGL_RS12375 - hypothetical protein, at 2,235,072 to 2,235,482
_RS12375
SGL_RS19925: SGL_RS19925 - IS630 transposase-related protein, at 2,235,561 to 2,235,920
_RS19925
SGL_RS18970: SGL_RS18970 - IS630 family transposase, at 2,235,830 to 2,236,408
_RS18970
SGL_RS12390: SGL_RS12390 - aromatic ring-hydroxylating dioxygenase subunit alpha, at 2,236,419 to 2,237,054
_RS12390
SGL_RS12395: SGL_RS12395 - S-methyl-5-thioribose-1-phosphate isomerase, at 2,237,134 to 2,238,189
_RS12395
Position (kb)
2236
2237
2238 Strain fitness (log2 ratio)
-1
0
1 at 2236.447 kb on - strand at 2236.462 kb on + strand at 2236.745 kb on - strand, within SGL_RS12390 at 2236.850 kb on + strand, within SGL_RS12390 at 2236.868 kb on - strand, within SGL_RS12390 at 2237.173 kb on - strand at 2237.211 kb on + strand at 2237.228 kb on + strand at 2237.246 kb on - strand, within SGL_RS12395 at 2237.257 kb on + strand, within SGL_RS12395 at 2237.257 kb on + strand, within SGL_RS12395 at 2237.258 kb on - strand, within SGL_RS12395 at 2237.265 kb on - strand, within SGL_RS12395 at 2237.276 kb on + strand, within SGL_RS12395 at 2237.294 kb on - strand, within SGL_RS12395 at 2237.470 kb on + strand, within SGL_RS12395 at 2237.499 kb on + strand, within SGL_RS12395 at 2237.507 kb on - strand, within SGL_RS12395 at 2237.692 kb on - strand, within SGL_RS12395 at 2237.693 kb on + strand, within SGL_RS12395 at 2237.715 kb on + strand, within SGL_RS12395 at 2237.715 kb on + strand, within SGL_RS12395 at 2237.876 kb on - strand, within SGL_RS12395
Per-strain Table
Position Strand Gene LocusTag Fraction Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library) remove 2,236,447 - -0.2 2,236,462 + -0.4 2,236,745 - SGL_RS12390 0.51 -0.2 2,236,850 + SGL_RS12390 0.68 -0.2 2,236,868 - SGL_RS12390 0.71 -1.2 2,237,173 - -0.1 2,237,211 + -0.3 2,237,228 + +0.2 2,237,246 - SGL_RS12395 0.11 +1.1 2,237,257 + SGL_RS12395 0.12 +0.2 2,237,257 + SGL_RS12395 0.12 -0.8 2,237,258 - SGL_RS12395 0.12 +0.9 2,237,265 - SGL_RS12395 0.12 -0.5 2,237,276 + SGL_RS12395 0.13 -0.2 2,237,294 - SGL_RS12395 0.15 +0.3 2,237,470 + SGL_RS12395 0.32 +0.6 2,237,499 + SGL_RS12395 0.35 +0.1 2,237,507 - SGL_RS12395 0.35 -0.2 2,237,692 - SGL_RS12395 0.53 -0.8 2,237,693 + SGL_RS12395 0.53 +0.6 2,237,715 + SGL_RS12395 0.55 -1.0 2,237,715 + SGL_RS12395 0.55 -0.2 2,237,876 - SGL_RS12395 0.70 +0.4
Or see this region's nucleotide sequence