Experiment: Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10145 and SGL_RS10150 are separated by 243 nucleotides SGL_RS10150 and SGL_RS10155 are separated by 181 nucleotides SGL_RS10155 and SGL_RS10160 are separated by 64 nucleotides
SGL_RS10145: SGL_RS10145 - cytochrome c, at 1,750,681 to 1,751,067
_RS10145
SGL_RS10150: SGL_RS10150 - 50S ribosomal protein L9, at 1,751,311 to 1,751,769
_RS10150
SGL_RS10155: SGL_RS10155 - hypothetical protein, at 1,751,951 to 1,752,826
_RS10155
SGL_RS10160: SGL_RS10160 - B12-binding domain-containing radical SAM protein, at 1,752,891 to 1,754,501
_RS10160
Position (kb)
1751
1752
1753 Strain fitness (log2 ratio)
-2
-1
0
1 at 1751.070 kb on - strand at 1751.070 kb on - strand at 1751.175 kb on - strand at 1751.408 kb on + strand, within SGL_RS10150 at 1751.478 kb on - strand, within SGL_RS10150 at 1751.649 kb on + strand, within SGL_RS10150 at 1751.936 kb on + strand at 1752.017 kb on - strand at 1752.045 kb on - strand, within SGL_RS10155 at 1752.330 kb on + strand, within SGL_RS10155 at 1752.424 kb on - strand, within SGL_RS10155 at 1752.435 kb on + strand, within SGL_RS10155 at 1752.451 kb on + strand, within SGL_RS10155 at 1752.461 kb on - strand, within SGL_RS10155 at 1752.461 kb on - strand, within SGL_RS10155 at 1752.692 kb on + strand, within SGL_RS10155 at 1752.710 kb on - strand, within SGL_RS10155 at 1752.948 kb on + strand at 1752.963 kb on - strand at 1753.034 kb on - strand at 1753.424 kb on - strand, within SGL_RS10160 at 1753.426 kb on + strand, within SGL_RS10160 at 1753.434 kb on - strand, within SGL_RS10160 at 1753.439 kb on - strand, within SGL_RS10160 at 1753.479 kb on - strand, within SGL_RS10160
Per-strain Table
Position Strand Gene LocusTag Fraction Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library) remove 1,751,070 - -0.1 1,751,070 - -0.1 1,751,175 - -0.5 1,751,408 + SGL_RS10150 0.21 +0.1 1,751,478 - SGL_RS10150 0.36 -0.0 1,751,649 + SGL_RS10150 0.74 -0.4 1,751,936 + -0.1 1,752,017 - -0.6 1,752,045 - SGL_RS10155 0.11 +0.3 1,752,330 + SGL_RS10155 0.43 -1.3 1,752,424 - SGL_RS10155 0.54 -2.0 1,752,435 + SGL_RS10155 0.55 -0.7 1,752,451 + SGL_RS10155 0.57 -0.7 1,752,461 - SGL_RS10155 0.58 +0.3 1,752,461 - SGL_RS10155 0.58 -1.1 1,752,692 + SGL_RS10155 0.85 -1.5 1,752,710 - SGL_RS10155 0.87 -0.6 1,752,948 + -0.0 1,752,963 - -0.3 1,753,034 - +1.6 1,753,424 - SGL_RS10160 0.33 +0.3 1,753,426 + SGL_RS10160 0.33 -0.1 1,753,434 - SGL_RS10160 0.34 +0.6 1,753,439 - SGL_RS10160 0.34 -1.0 1,753,479 - SGL_RS10160 0.36 -0.6
Or see this region's nucleotide sequence