Experiment: Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07325 and SGL_RS18770 are separated by 85 nucleotides SGL_RS18770 and SGL_RS07330 are separated by 45 nucleotides SGL_RS07330 and SGL_RS07335 are separated by 181 nucleotides SGL_RS07335 and SGL_RS07340 are separated by 127 nucleotides
SGL_RS07325: SGL_RS07325 - photosystem II reaction center protein PsbH, at 1,167,053 to 1,167,247
_RS07325
SGL_RS18770: SGL_RS18770 - photosystem II reaction center protein PsbN, at 1,167,333 to 1,167,464
_RS18770
SGL_RS07330: SGL_RS07330 - YciI family protein, at 1,167,510 to 1,167,779
_RS07330
SGL_RS07335: SGL_RS07335 - hypothetical protein, at 1,167,961 to 1,168,950
_RS07335
SGL_RS07340: SGL_RS07340 - TRAP transporter substrate-binding protein, at 1,169,078 to 1,170,187
_RS07340
Position (kb) 1167
1168
1169 Strain fitness (log2 ratio) -1
0
1 at 1167.068 kb on - strand at 1167.077 kb on + strand, within SGL_RS07325 at 1167.331 kb on - strand at 1167.346 kb on - strand at 1167.429 kb on + strand, within SGL_RS18770 at 1167.429 kb on + strand, within SGL_RS18770 at 1167.588 kb on + strand, within SGL_RS07330 at 1167.653 kb on + strand, within SGL_RS07330 at 1167.699 kb on + strand, within SGL_RS07330 at 1167.772 kb on - strand at 1167.781 kb on + strand at 1167.896 kb on - strand at 1167.928 kb on - strand at 1167.963 kb on + strand at 1168.028 kb on - strand at 1168.256 kb on + strand, within SGL_RS07335 at 1168.815 kb on - strand, within SGL_RS07335 at 1168.899 kb on - strand at 1168.916 kb on - strand at 1168.917 kb on - strand at 1169.274 kb on + strand, within SGL_RS07340 at 1169.874 kb on + strand, within SGL_RS07340
Per-strain Table
Position Strand Gene LocusTag Fraction Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library) remove 1,167,068 - +0.2 1,167,077 + SGL_RS07325 0.12 -1.6 1,167,331 - +0.2 1,167,346 - -0.1 1,167,429 + SGL_RS18770 0.73 -0.2 1,167,429 + SGL_RS18770 0.73 +1.1 1,167,588 + SGL_RS07330 0.29 +0.1 1,167,653 + SGL_RS07330 0.53 -0.1 1,167,699 + SGL_RS07330 0.70 +0.3 1,167,772 - +0.4 1,167,781 + +0.1 1,167,896 - -0.1 1,167,928 - +0.1 1,167,963 + +1.5 1,168,028 - -0.0 1,168,256 + SGL_RS07335 0.30 -0.5 1,168,815 - SGL_RS07335 0.86 -0.6 1,168,899 - -0.4 1,168,916 - -0.7 1,168,917 - -0.6 1,169,274 + SGL_RS07340 0.18 -0.2 1,169,874 + SGL_RS07340 0.72 -0.9
Or see this region's nucleotide sequence