Experiment: Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05970 and SGL_RS05975 are separated by 153 nucleotides SGL_RS05975 and SGL_RS05980 are separated by 28 nucleotides SGL_RS05980 and SGL_RS05985 are separated by 27 nucleotides
SGL_RS05970: SGL_RS05970 - DUF533 domain-containing protein, at 874,693 to 875,361
_RS05970
SGL_RS05975: SGL_RS05975 - DUF3531 family protein, at 875,515 to 875,979
_RS05975
SGL_RS05980: SGL_RS05980 - hypothetical protein, at 876,008 to 877,297
_RS05980
SGL_RS05985: SGL_RS05985 - DNA-processing protein DprA, at 877,325 to 878,521
_RS05985
Position (kb) 876
877
878 Strain fitness (log2 ratio) -2
-1
0
1
2 at 875.130 kb on - strand, within SGL_RS05970 at 875.201 kb on - strand, within SGL_RS05970 at 875.216 kb on + strand, within SGL_RS05970 at 875.341 kb on - strand at 875.603 kb on + strand, within SGL_RS05975 at 875.756 kb on + strand, within SGL_RS05975 at 875.756 kb on + strand, within SGL_RS05975 at 876.126 kb on - strand at 876.133 kb on + strand at 876.139 kb on + strand, within SGL_RS05980 at 876.392 kb on + strand, within SGL_RS05980 at 876.404 kb on + strand, within SGL_RS05980 at 876.446 kb on + strand, within SGL_RS05980 at 876.563 kb on - strand, within SGL_RS05980 at 876.684 kb on - strand, within SGL_RS05980 at 876.725 kb on + strand, within SGL_RS05980 at 876.804 kb on + strand, within SGL_RS05980 at 876.913 kb on + strand, within SGL_RS05980 at 876.964 kb on + strand, within SGL_RS05980 at 877.040 kb on - strand, within SGL_RS05980 at 877.169 kb on + strand at 877.202 kb on - strand at 877.366 kb on + strand at 877.388 kb on - strand at 877.411 kb on - strand at 877.628 kb on + strand, within SGL_RS05985 at 877.655 kb on - strand, within SGL_RS05985 at 877.805 kb on - strand, within SGL_RS05985 at 878.008 kb on + strand, within SGL_RS05985 at 878.011 kb on - strand, within SGL_RS05985 at 878.016 kb on - strand, within SGL_RS05985 at 878.038 kb on + strand, within SGL_RS05985 at 878.059 kb on - strand, within SGL_RS05985 at 878.059 kb on - strand, within SGL_RS05985 at 878.093 kb on + strand, within SGL_RS05985 at 878.101 kb on - strand, within SGL_RS05985 at 878.101 kb on - strand, within SGL_RS05985
Per-strain Table
Position Strand Gene LocusTag Fraction Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library) remove 875,130 - SGL_RS05970 0.65 +2.5 875,201 - SGL_RS05970 0.76 -0.1 875,216 + SGL_RS05970 0.78 -1.0 875,341 - -0.0 875,603 + SGL_RS05975 0.19 -0.3 875,756 + SGL_RS05975 0.52 -0.0 875,756 + SGL_RS05975 0.52 +0.1 876,126 - -1.7 876,133 + -1.3 876,139 + SGL_RS05980 0.10 +1.5 876,392 + SGL_RS05980 0.30 -1.9 876,404 + SGL_RS05980 0.31 -0.5 876,446 + SGL_RS05980 0.34 -0.3 876,563 - SGL_RS05980 0.43 -0.3 876,684 - SGL_RS05980 0.52 +0.1 876,725 + SGL_RS05980 0.56 -2.3 876,804 + SGL_RS05980 0.62 -1.5 876,913 + SGL_RS05980 0.70 -0.1 876,964 + SGL_RS05980 0.74 -1.7 877,040 - SGL_RS05980 0.80 -1.0 877,169 + -1.3 877,202 - -0.7 877,366 + -0.2 877,388 - -0.1 877,411 - -0.3 877,628 + SGL_RS05985 0.25 -0.2 877,655 - SGL_RS05985 0.28 +0.3 877,805 - SGL_RS05985 0.40 -0.3 878,008 + SGL_RS05985 0.57 +0.6 878,011 - SGL_RS05985 0.57 +0.0 878,016 - SGL_RS05985 0.58 +0.0 878,038 + SGL_RS05985 0.60 +0.2 878,059 - SGL_RS05985 0.61 -0.2 878,059 - SGL_RS05985 0.61 -0.6 878,093 + SGL_RS05985 0.64 -0.3 878,101 - SGL_RS05985 0.65 -0.4 878,101 - SGL_RS05985 0.65 +0.8
Or see this region's nucleotide sequence