Experiment: Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05415 and SGL_RS05420 are separated by 156 nucleotides SGL_RS05420 and SGL_RS05425 are separated by 253 nucleotides
SGL_RS05415: SGL_RS05415 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 757,671 to 758,753
_RS05415
SGL_RS05420: SGL_RS05420 - hypothetical protein, at 758,910 to 761,228
_RS05420
SGL_RS05425: SGL_RS05425 - ABC transporter ATP-binding protein, at 761,482 to 763,263
_RS05425
Position (kb) 758
759
760
761
762 Strain fitness (log2 ratio) -1
0
1 at 758.029 kb on + strand, within SGL_RS05415 at 758.328 kb on - strand, within SGL_RS05415 at 758.362 kb on + strand, within SGL_RS05415 at 758.517 kb on + strand, within SGL_RS05415 at 759.129 kb on - strand at 759.129 kb on - strand at 759.177 kb on + strand, within SGL_RS05420 at 759.248 kb on - strand, within SGL_RS05420 at 759.262 kb on + strand, within SGL_RS05420 at 759.274 kb on - strand, within SGL_RS05420 at 759.345 kb on + strand, within SGL_RS05420 at 759.352 kb on + strand, within SGL_RS05420 at 759.534 kb on - strand, within SGL_RS05420 at 759.827 kb on + strand, within SGL_RS05420 at 759.953 kb on + strand, within SGL_RS05420 at 759.981 kb on - strand, within SGL_RS05420 at 760.049 kb on - strand, within SGL_RS05420 at 760.322 kb on + strand, within SGL_RS05420 at 760.486 kb on - strand, within SGL_RS05420 at 760.628 kb on - strand, within SGL_RS05420 at 760.651 kb on - strand, within SGL_RS05420 at 760.651 kb on - strand, within SGL_RS05420 at 760.737 kb on + strand, within SGL_RS05420 at 760.994 kb on - strand, within SGL_RS05420 at 761.022 kb on + strand at 761.023 kb on + strand at 761.023 kb on + strand at 761.108 kb on - strand at 761.334 kb on - strand at 761.397 kb on + strand at 761.429 kb on + strand at 761.437 kb on - strand at 761.439 kb on - strand at 761.554 kb on + strand at 761.841 kb on - strand, within SGL_RS05425 at 761.957 kb on - strand, within SGL_RS05425 at 761.989 kb on + strand, within SGL_RS05425 at 762.068 kb on + strand, within SGL_RS05425 at 762.079 kb on + strand, within SGL_RS05425
Per-strain Table
Position Strand Gene LocusTag Fraction Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library) remove 758,029 + SGL_RS05415 0.33 +0.1 758,328 - SGL_RS05415 0.61 +0.5 758,362 + SGL_RS05415 0.64 +0.2 758,517 + SGL_RS05415 0.78 -0.4 759,129 - -0.0 759,129 - -0.0 759,177 + SGL_RS05420 0.12 +0.9 759,248 - SGL_RS05420 0.15 -0.2 759,262 + SGL_RS05420 0.15 +0.3 759,274 - SGL_RS05420 0.16 -1.1 759,345 + SGL_RS05420 0.19 -0.3 759,352 + SGL_RS05420 0.19 +0.0 759,534 - SGL_RS05420 0.27 +0.3 759,827 + SGL_RS05420 0.40 -0.2 759,953 + SGL_RS05420 0.45 +0.4 759,981 - SGL_RS05420 0.46 +0.5 760,049 - SGL_RS05420 0.49 -0.1 760,322 + SGL_RS05420 0.61 -0.2 760,486 - SGL_RS05420 0.68 -0.2 760,628 - SGL_RS05420 0.74 -0.1 760,651 - SGL_RS05420 0.75 -0.2 760,651 - SGL_RS05420 0.75 -0.5 760,737 + SGL_RS05420 0.79 -0.2 760,994 - SGL_RS05420 0.90 -0.2 761,022 + -0.4 761,023 + -0.1 761,023 + +0.5 761,108 - -1.1 761,334 - -0.2 761,397 + +0.5 761,429 + +0.4 761,437 - +0.0 761,439 - -0.2 761,554 + -1.7 761,841 - SGL_RS05425 0.20 -0.2 761,957 - SGL_RS05425 0.27 +0.7 761,989 + SGL_RS05425 0.28 -0.0 762,068 + SGL_RS05425 0.33 +0.3 762,079 + SGL_RS05425 0.34 -0.5
Or see this region's nucleotide sequence