Experiment: Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05345 and SGL_RS05350 are separated by 214 nucleotides SGL_RS05350 and SGL_RS05355 overlap by 4 nucleotides
SGL_RS05345: SGL_RS05345 - DUF2301 domain-containing membrane protein, at 746,303 to 747,019
_RS05345
SGL_RS05350: SGL_RS05350 - NADH-quinone oxidoreductase subunit K, at 747,234 to 747,578
_RS05350
SGL_RS05355: SGL_RS05355 - cation:proton antiporter, at 747,575 to 749,038
_RS05355
Position (kb) 747
748 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 746.280 kb on + strand at 746.381 kb on + strand, within SGL_RS05345 at 746.394 kb on - strand, within SGL_RS05345 at 746.461 kb on + strand, within SGL_RS05345 at 746.620 kb on - strand, within SGL_RS05345 at 746.713 kb on + strand, within SGL_RS05345 at 746.733 kb on + strand, within SGL_RS05345 at 746.775 kb on - strand, within SGL_RS05345 at 746.828 kb on + strand, within SGL_RS05345 at 747.050 kb on - strand at 747.175 kb on - strand at 747.235 kb on + strand at 747.394 kb on + strand, within SGL_RS05350 at 747.433 kb on - strand, within SGL_RS05350 at 747.453 kb on + strand, within SGL_RS05350 at 747.453 kb on + strand, within SGL_RS05350 at 747.463 kb on + strand, within SGL_RS05350 at 747.511 kb on + strand, within SGL_RS05350 at 747.519 kb on - strand, within SGL_RS05350 at 747.564 kb on + strand at 748.014 kb on + strand, within SGL_RS05355 at 748.019 kb on + strand, within SGL_RS05355 at 748.022 kb on - strand, within SGL_RS05355 at 748.053 kb on + strand, within SGL_RS05355 at 748.089 kb on - strand, within SGL_RS05355 at 748.095 kb on + strand, within SGL_RS05355 at 748.095 kb on + strand, within SGL_RS05355 at 748.315 kb on - strand, within SGL_RS05355 at 748.317 kb on + strand, within SGL_RS05355 at 748.317 kb on + strand, within SGL_RS05355 at 748.387 kb on + strand, within SGL_RS05355 at 748.395 kb on - strand, within SGL_RS05355 at 748.395 kb on - strand, within SGL_RS05355 at 748.395 kb on - strand, within SGL_RS05355 at 748.395 kb on - strand, within SGL_RS05355 at 748.512 kb on + strand, within SGL_RS05355 at 748.547 kb on + strand, within SGL_RS05355
Per-strain Table
Position Strand Gene LocusTag Fraction Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library) remove 746,280 + +0.2 746,381 + SGL_RS05345 0.11 -0.4 746,394 - SGL_RS05345 0.13 -0.0 746,461 + SGL_RS05345 0.22 +0.4 746,620 - SGL_RS05345 0.44 -0.3 746,713 + SGL_RS05345 0.57 -0.7 746,733 + SGL_RS05345 0.60 -0.4 746,775 - SGL_RS05345 0.66 -0.1 746,828 + SGL_RS05345 0.73 -0.0 747,050 - -0.2 747,175 - -0.4 747,235 + -0.3 747,394 + SGL_RS05350 0.46 -0.6 747,433 - SGL_RS05350 0.58 -1.0 747,453 + SGL_RS05350 0.63 -1.1 747,453 + SGL_RS05350 0.63 -0.6 747,463 + SGL_RS05350 0.66 +0.4 747,511 + SGL_RS05350 0.80 -0.1 747,519 - SGL_RS05350 0.83 -0.9 747,564 + -0.1 748,014 + SGL_RS05355 0.30 -2.2 748,019 + SGL_RS05355 0.30 -1.3 748,022 - SGL_RS05355 0.31 -2.7 748,053 + SGL_RS05355 0.33 -1.5 748,089 - SGL_RS05355 0.35 -1.3 748,095 + SGL_RS05355 0.36 -1.7 748,095 + SGL_RS05355 0.36 -1.5 748,315 - SGL_RS05355 0.51 -0.7 748,317 + SGL_RS05355 0.51 -0.8 748,317 + SGL_RS05355 0.51 -1.1 748,387 + SGL_RS05355 0.55 -0.4 748,395 - SGL_RS05355 0.56 -0.8 748,395 - SGL_RS05355 0.56 -0.8 748,395 - SGL_RS05355 0.56 -1.4 748,395 - SGL_RS05355 0.56 -1.4 748,512 + SGL_RS05355 0.64 -2.7 748,547 + SGL_RS05355 0.66 -2.8
Or see this region's nucleotide sequence