Experiment: Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00465 and SGL_RS00470 are separated by 220 nucleotides SGL_RS00470 and SGL_RS00475 overlap by 8 nucleotides SGL_RS00475 and SGL_RS00480 are separated by 120 nucleotides SGL_RS00480 and SGL_RS00485 are separated by 297 nucleotides
SGL_RS00465: SGL_RS00465 - hypothetical protein, at 95,697 to 95,903
_RS00465
SGL_RS00470: SGL_RS00470 - DUF6088 family protein, at 96,124 to 96,561
_RS00470
SGL_RS00475: SGL_RS00475 - nucleotidyl transferase AbiEii/AbiGii toxin family protein, at 96,554 to 97,156
_RS00475
SGL_RS00480: SGL_RS00480 - hypothetical protein, at 97,277 to 97,492
_RS00480
SGL_RS00485: SGL_RS00485 - arsenate reductase family protein, at 97,790 to 98,212
_RS00485
Position (kb) 96
97
98 Strain fitness (log2 ratio) -1
0
1 at 95.693 kb on - strand at 95.703 kb on - strand at 95.915 kb on + strand at 95.923 kb on - strand at 95.954 kb on - strand at 95.963 kb on + strand at 96.268 kb on + strand, within SGL_RS00470 at 96.286 kb on - strand, within SGL_RS00470 at 96.318 kb on - strand, within SGL_RS00470 at 96.359 kb on - strand, within SGL_RS00470 at 96.531 kb on - strand at 96.553 kb on - strand at 96.564 kb on + strand at 96.623 kb on - strand, within SGL_RS00475 at 96.645 kb on - strand, within SGL_RS00475 at 96.981 kb on + strand, within SGL_RS00475 at 97.098 kb on + strand at 97.119 kb on + strand at 97.411 kb on + strand, within SGL_RS00480 at 97.411 kb on + strand, within SGL_RS00480 at 97.451 kb on + strand, within SGL_RS00480 at 97.563 kb on - strand at 97.601 kb on + strand at 97.631 kb on + strand at 97.646 kb on + strand at 97.673 kb on - strand at 98.058 kb on + strand, within SGL_RS00485
Per-strain Table
Position Strand Gene LocusTag Fraction Synechocystis_PCC6803_ML3a diversity barseq (outgrowth of original library) remove 95,693 - +0.7 95,703 - -0.1 95,915 + -1.4 95,923 - +0.0 95,954 - +0.8 95,963 + -0.5 96,268 + SGL_RS00470 0.33 -0.1 96,286 - SGL_RS00470 0.37 -0.4 96,318 - SGL_RS00470 0.44 +0.4 96,359 - SGL_RS00470 0.54 +0.1 96,531 - -0.1 96,553 - -0.1 96,564 + +0.2 96,623 - SGL_RS00475 0.11 -0.7 96,645 - SGL_RS00475 0.15 -0.4 96,981 + SGL_RS00475 0.71 -0.5 97,098 + +0.2 97,119 + +0.0 97,411 + SGL_RS00480 0.62 -0.6 97,411 + SGL_RS00480 0.62 -0.2 97,451 + SGL_RS00480 0.81 -0.1 97,563 - -0.0 97,601 + +0.1 97,631 + -0.5 97,646 + +0.2 97,673 - +0.2 98,058 + SGL_RS00485 0.63 +0.1
Or see this region's nucleotide sequence