Experiment: growth in full media supplemented with 3 .M Cl2Co but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15325 and SGL_RS15330 are separated by 245 nucleotides SGL_RS15330 and SGL_RS15335 are separated by 2 nucleotides SGL_RS15335 and SGL_RS15340 are separated by 171 nucleotides
SGL_RS15325: SGL_RS15325 - N-acetyl-gamma-glutamyl-phosphate reductase, at 2,889,265 to 2,890,320
_RS15325
SGL_RS15330: SGL_RS15330 - imidazole glycerol phosphate synthase subunit HisH, at 2,890,566 to 2,891,198
_RS15330
SGL_RS15335: SGL_RS15335 - threonine--tRNA ligase, at 2,891,201 to 2,893,012
_RS15335
SGL_RS15340: SGL_RS15340 - Hsp70 family protein, at 2,893,184 to 2,894,779
_RS15340
Position (kb)
2891
2892
2893
2894 Strain fitness (log2 ratio)
-1
0
1 at 2890.469 kb on + strand at 2890.542 kb on + strand at 2890.550 kb on - strand at 2890.689 kb on - strand, within SGL_RS15330 at 2893.072 kb on + strand at 2893.100 kb on - strand at 2893.138 kb on + strand at 2893.143 kb on + strand at 2893.151 kb on - strand at 2893.194 kb on - strand at 2893.277 kb on - strand at 2893.289 kb on - strand at 2893.481 kb on - strand, within SGL_RS15340 at 2893.499 kb on + strand, within SGL_RS15340 at 2893.630 kb on + strand, within SGL_RS15340 at 2893.642 kb on + strand, within SGL_RS15340 at 2893.684 kb on + strand, within SGL_RS15340 at 2893.684 kb on + strand, within SGL_RS15340 at 2893.687 kb on - strand, within SGL_RS15340 at 2893.692 kb on - strand, within SGL_RS15340 at 2893.692 kb on - strand, within SGL_RS15340 at 2893.757 kb on + strand, within SGL_RS15340 at 2893.775 kb on + strand, within SGL_RS15340 at 2893.941 kb on + strand, within SGL_RS15340 at 2893.942 kb on - strand, within SGL_RS15340 at 2893.942 kb on - strand, within SGL_RS15340
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 3 .M Cl2Co but diluted to prevent light attenuation remove 2,890,469 + +0.4 2,890,542 + -1.5 2,890,550 - -1.7 2,890,689 - SGL_RS15330 0.19 +0.8 2,893,072 + -0.2 2,893,100 - +0.8 2,893,138 + -0.2 2,893,143 + +0.2 2,893,151 - -0.1 2,893,194 - -0.4 2,893,277 - +0.3 2,893,289 - -0.6 2,893,481 - SGL_RS15340 0.19 -0.7 2,893,499 + SGL_RS15340 0.20 +0.0 2,893,630 + SGL_RS15340 0.28 +1.4 2,893,642 + SGL_RS15340 0.29 -0.5 2,893,684 + SGL_RS15340 0.31 -0.6 2,893,684 + SGL_RS15340 0.31 -0.2 2,893,687 - SGL_RS15340 0.32 -0.2 2,893,692 - SGL_RS15340 0.32 +0.2 2,893,692 - SGL_RS15340 0.32 -0.1 2,893,757 + SGL_RS15340 0.36 -0.1 2,893,775 + SGL_RS15340 0.37 -0.1 2,893,941 + SGL_RS15340 0.47 -0.2 2,893,942 - SGL_RS15340 0.47 -0.2 2,893,942 - SGL_RS15340 0.47 -0.1
Or see this region's nucleotide sequence