Experiment: growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15845 and SGL_RS15850 are separated by 168 nucleotides SGL_RS15850 and SGL_RS15855 are separated by 45 nucleotides SGL_RS15855 and SGL_RS15860 are separated by 9 nucleotides SGL_RS15860 and SGL_RS15865 are separated by 57 nucleotides SGL_RS15865 and SGL_RS15870 are separated by 76 nucleotides
SGL_RS15845: SGL_RS15845 - polyphosphate kinase 1, at 3,004,454 to 3,006,640
_RS15845
SGL_RS15850: SGL_RS15850 - hypothetical protein, at 3,006,809 to 3,007,003
_RS15850
SGL_RS15855: SGL_RS15855 - hypothetical protein, at 3,007,049 to 3,007,270
_RS15855
SGL_RS15860: SGL_RS15860 - cell division topological specificity factor MinE, at 3,007,280 to 3,007,573
_RS15860
SGL_RS15865: SGL_RS15865 - septum site-determining protein MinD, at 3,007,631 to 3,008,431
_RS15865
SGL_RS15870: SGL_RS15870 - septum site-determining protein MinC, at 3,008,508 to 3,009,251
_RS15870
Position (kb)
3007
3008
3009 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3006.631 kb on - strand at 3006.635 kb on + strand at 3006.643 kb on - strand at 3006.693 kb on + strand at 3006.693 kb on + strand at 3006.694 kb on + strand at 3006.701 kb on - strand at 3006.701 kb on - strand at 3007.024 kb on + strand at 3007.106 kb on - strand, within SGL_RS15855 at 3007.168 kb on - strand, within SGL_RS15855 at 3007.220 kb on + strand, within SGL_RS15855 at 3007.585 kb on - strand at 3007.599 kb on - strand at 3007.635 kb on + strand at 3007.679 kb on - strand at 3007.679 kb on - strand at 3007.783 kb on - strand, within SGL_RS15865 at 3007.985 kb on - strand, within SGL_RS15865 at 3007.996 kb on + strand, within SGL_RS15865 at 3008.348 kb on + strand, within SGL_RS15865 at 3008.416 kb on + strand at 3008.472 kb on - strand at 3008.627 kb on - strand, within SGL_RS15870 at 3008.708 kb on - strand, within SGL_RS15870 at 3008.763 kb on - strand, within SGL_RS15870 at 3008.784 kb on + strand, within SGL_RS15870 at 3008.836 kb on - strand, within SGL_RS15870 at 3008.836 kb on - strand, within SGL_RS15870 at 3008.866 kb on - strand, within SGL_RS15870 at 3008.896 kb on + strand, within SGL_RS15870 at 3008.909 kb on - strand, within SGL_RS15870 at 3008.958 kb on + strand, within SGL_RS15870 at 3008.970 kb on + strand, within SGL_RS15870 at 3009.143 kb on + strand, within SGL_RS15870 at 3009.143 kb on + strand, within SGL_RS15870 at 3009.151 kb on - strand, within SGL_RS15870 at 3009.151 kb on - strand, within SGL_RS15870 at 3009.229 kb on + strand at 3009.229 kb on + strand at 3009.237 kb on - strand at 3009.291 kb on - strand at 3009.291 kb on - strand at 3009.291 kb on - strand at 3009.320 kb on + strand at 3009.328 kb on - strand at 3009.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation remove 3,006,631 - -0.1 3,006,635 + +0.3 3,006,643 - +0.8 3,006,693 + +0.0 3,006,693 + +1.3 3,006,694 + +0.2 3,006,701 - +0.6 3,006,701 - -0.2 3,007,024 + +0.4 3,007,106 - SGL_RS15855 0.26 -0.2 3,007,168 - SGL_RS15855 0.54 -0.4 3,007,220 + SGL_RS15855 0.77 -0.2 3,007,585 - +0.5 3,007,599 - -0.3 3,007,635 + -1.0 3,007,679 - -0.6 3,007,679 - -1.5 3,007,783 - SGL_RS15865 0.19 -1.6 3,007,985 - SGL_RS15865 0.44 -1.6 3,007,996 + SGL_RS15865 0.46 -0.7 3,008,348 + SGL_RS15865 0.90 -2.9 3,008,416 + -0.9 3,008,472 - -0.3 3,008,627 - SGL_RS15870 0.16 -2.0 3,008,708 - SGL_RS15870 0.27 -0.3 3,008,763 - SGL_RS15870 0.34 +0.4 3,008,784 + SGL_RS15870 0.37 -0.3 3,008,836 - SGL_RS15870 0.44 -1.1 3,008,836 - SGL_RS15870 0.44 -1.3 3,008,866 - SGL_RS15870 0.48 -1.0 3,008,896 + SGL_RS15870 0.52 -0.6 3,008,909 - SGL_RS15870 0.54 -0.7 3,008,958 + SGL_RS15870 0.60 -1.0 3,008,970 + SGL_RS15870 0.62 -0.7 3,009,143 + SGL_RS15870 0.85 -0.9 3,009,143 + SGL_RS15870 0.85 -1.9 3,009,151 - SGL_RS15870 0.86 -1.2 3,009,151 - SGL_RS15870 0.86 -1.3 3,009,229 + -0.3 3,009,229 + -2.0 3,009,237 - -0.9 3,009,291 - -1.6 3,009,291 - -0.7 3,009,291 - +0.0 3,009,320 + -0.5 3,009,328 - -0.2 3,009,384 - -0.1
Or see this region's nucleotide sequence