Experiment: growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13500 and SGL_RS13505 are separated by 100 nucleotides SGL_RS13505 and SGL_RS13510 are separated by 65 nucleotides SGL_RS13510 and SGL_RS13515 are separated by 226 nucleotides SGL_RS13515 and SGL_RS13520 are separated by 225 nucleotides
SGL_RS13500: SGL_RS13500 - ATP-dependent DNA helicase RecG, at 2,497,726 to 2,500,221
_RS13500
SGL_RS13505: SGL_RS13505 - signal peptide peptidase SppA, at 2,500,322 to 2,501,155
_RS13505
SGL_RS13510: SGL_RS13510 - transglycosylase domain-containing protein, at 2,501,221 to 2,503,878
_RS13510
SGL_RS13515: SGL_RS13515 - tRNA-Ser, at 2,504,105 to 2,504,189
_RS13515
SGL_RS13520: SGL_RS13520 - glutamine-hydrolyzing GMP synthase, at 2,504,415 to 2,506,043
_RS13520
Position (kb)
2501
2502
2503
2504 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2500.417 kb on - strand, within SGL_RS13505 at 2500.462 kb on - strand, within SGL_RS13505 at 2500.487 kb on + strand, within SGL_RS13505 at 2500.495 kb on - strand, within SGL_RS13505 at 2500.580 kb on + strand, within SGL_RS13505 at 2500.813 kb on + strand, within SGL_RS13505 at 2500.997 kb on - strand, within SGL_RS13505 at 2501.012 kb on - strand, within SGL_RS13505 at 2501.014 kb on + strand, within SGL_RS13505 at 2501.127 kb on + strand at 2501.201 kb on - strand at 2501.251 kb on + strand at 2501.318 kb on + strand at 2501.437 kb on - strand at 2501.466 kb on - strand at 2501.554 kb on - strand, within SGL_RS13510 at 2501.958 kb on - strand, within SGL_RS13510 at 2502.196 kb on - strand, within SGL_RS13510 at 2502.197 kb on - strand, within SGL_RS13510 at 2502.290 kb on + strand, within SGL_RS13510 at 2502.426 kb on - strand, within SGL_RS13510 at 2502.500 kb on - strand, within SGL_RS13510 at 2502.655 kb on + strand, within SGL_RS13510 at 2502.753 kb on + strand, within SGL_RS13510 at 2502.768 kb on - strand, within SGL_RS13510 at 2502.804 kb on + strand, within SGL_RS13510 at 2502.879 kb on - strand, within SGL_RS13510 at 2503.013 kb on + strand, within SGL_RS13510 at 2503.221 kb on - strand, within SGL_RS13510 at 2503.466 kb on + strand, within SGL_RS13510 at 2503.488 kb on - strand, within SGL_RS13510 at 2503.586 kb on + strand, within SGL_RS13510 at 2503.752 kb on - strand at 2504.042 kb on + strand at 2504.249 kb on - strand at 2504.259 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation remove 2,500,417 - SGL_RS13505 0.11 -0.4 2,500,462 - SGL_RS13505 0.17 -0.2 2,500,487 + SGL_RS13505 0.20 +0.1 2,500,495 - SGL_RS13505 0.21 -0.7 2,500,580 + SGL_RS13505 0.31 -0.4 2,500,813 + SGL_RS13505 0.59 +0.0 2,500,997 - SGL_RS13505 0.81 -0.1 2,501,012 - SGL_RS13505 0.83 +0.2 2,501,014 + SGL_RS13505 0.83 -0.3 2,501,127 + -0.5 2,501,201 - +0.0 2,501,251 + -0.4 2,501,318 + -0.1 2,501,437 - +1.0 2,501,466 - -0.2 2,501,554 - SGL_RS13510 0.13 -0.9 2,501,958 - SGL_RS13510 0.28 -2.9 2,502,196 - SGL_RS13510 0.37 -1.4 2,502,197 - SGL_RS13510 0.37 -2.4 2,502,290 + SGL_RS13510 0.40 -1.3 2,502,426 - SGL_RS13510 0.45 -0.5 2,502,500 - SGL_RS13510 0.48 -2.7 2,502,655 + SGL_RS13510 0.54 -3.7 2,502,753 + SGL_RS13510 0.58 -1.3 2,502,768 - SGL_RS13510 0.58 -2.3 2,502,804 + SGL_RS13510 0.60 -1.6 2,502,879 - SGL_RS13510 0.62 -1.6 2,503,013 + SGL_RS13510 0.67 -3.8 2,503,221 - SGL_RS13510 0.75 -2.4 2,503,466 + SGL_RS13510 0.84 -3.1 2,503,488 - SGL_RS13510 0.85 -1.6 2,503,586 + SGL_RS13510 0.89 -2.9 2,503,752 - -1.3 2,504,042 + +0.3 2,504,249 - -0.0 2,504,259 - +0.0
Or see this region's nucleotide sequence