Experiment: growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12535 and SGL_RS12540 are separated by 40 nucleotides SGL_RS12540 and SGL_RS12545 are separated by 460 nucleotides
SGL_RS12535: SGL_RS12535 - radical SAM protein, at 2,267,490 to 2,269,085
_RS12535
SGL_RS12540: SGL_RS12540 - RNA polymerase sigma factor, RpoD/SigA family, at 2,269,126 to 2,270,163
_RS12540
SGL_RS12545: SGL_RS12545 - GTPase Era, at 2,270,624 to 2,271,571
_RS12545
Position (kb)
2269
2270
2271 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2268.172 kb on + strand, within SGL_RS12535 at 2268.277 kb on + strand, within SGL_RS12535 at 2268.299 kb on + strand, within SGL_RS12535 at 2268.448 kb on - strand, within SGL_RS12535 at 2268.450 kb on + strand, within SGL_RS12535 at 2268.458 kb on - strand, within SGL_RS12535 at 2268.458 kb on - strand, within SGL_RS12535 at 2268.619 kb on + strand, within SGL_RS12535 at 2268.852 kb on - strand, within SGL_RS12535 at 2268.917 kb on + strand, within SGL_RS12535 at 2268.998 kb on + strand at 2269.016 kb on - strand at 2269.016 kb on - strand at 2269.445 kb on - strand, within SGL_RS12540 at 2269.513 kb on - strand, within SGL_RS12540 at 2269.616 kb on + strand, within SGL_RS12540 at 2269.621 kb on + strand, within SGL_RS12540 at 2269.750 kb on - strand, within SGL_RS12540 at 2269.859 kb on - strand, within SGL_RS12540 at 2270.051 kb on - strand, within SGL_RS12540 at 2270.087 kb on + strand at 2270.197 kb on - strand at 2270.204 kb on + strand at 2270.204 kb on + strand at 2270.214 kb on + strand at 2270.317 kb on + strand at 2270.319 kb on - strand at 2270.463 kb on + strand at 2270.554 kb on - strand at 2270.710 kb on - strand at 2271.062 kb on - strand, within SGL_RS12545 at 2271.118 kb on - strand, within SGL_RS12545 at 2271.157 kb on - strand, within SGL_RS12545
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation remove 2,268,172 + SGL_RS12535 0.43 +0.9 2,268,277 + SGL_RS12535 0.49 -0.6 2,268,299 + SGL_RS12535 0.51 -0.0 2,268,448 - SGL_RS12535 0.60 +0.2 2,268,450 + SGL_RS12535 0.60 -0.0 2,268,458 - SGL_RS12535 0.61 +0.2 2,268,458 - SGL_RS12535 0.61 +0.7 2,268,619 + SGL_RS12535 0.71 +1.3 2,268,852 - SGL_RS12535 0.85 -0.2 2,268,917 + SGL_RS12535 0.89 +0.5 2,268,998 + +0.1 2,269,016 - +0.4 2,269,016 - -0.9 2,269,445 - SGL_RS12540 0.31 -2.1 2,269,513 - SGL_RS12540 0.37 -2.3 2,269,616 + SGL_RS12540 0.47 -2.5 2,269,621 + SGL_RS12540 0.48 -2.1 2,269,750 - SGL_RS12540 0.60 -2.8 2,269,859 - SGL_RS12540 0.71 -2.2 2,270,051 - SGL_RS12540 0.89 -3.2 2,270,087 + -2.5 2,270,197 - -0.9 2,270,204 + -1.4 2,270,204 + -1.8 2,270,214 + -1.9 2,270,317 + -0.9 2,270,319 - -0.5 2,270,463 + -0.1 2,270,554 - +0.9 2,270,710 - -0.9 2,271,062 - SGL_RS12545 0.46 -0.1 2,271,118 - SGL_RS12545 0.52 -1.3 2,271,157 - SGL_RS12545 0.56 -0.1
Or see this region's nucleotide sequence