Strain Fitness in Synechocystis sp000284455 PCC 6803 around SGL_RS12515

Experiment: growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSGL_RS12510 and SGL_RS12515 overlap by 20 nucleotidesSGL_RS12515 and SGL_RS12520 are separated by 200 nucleotidesSGL_RS12520 and SGL_RS12525 are separated by 159 nucleotides SGL_RS12510: SGL_RS12510 - lipoyl(octanoyl) transferase LipB, at 2,263,827 to 2,264,510 _RS12510 SGL_RS12515: SGL_RS12515 - hypothetical protein, at 2,264,491 to 2,264,781 _RS12515 SGL_RS12520: SGL_RS12520 - tRNA-Lys, at 2,264,982 to 2,265,053 _RS12520 SGL_RS12525: SGL_RS12525 - glycogen synthase GlgA, at 2,265,213 to 2,266,646 _RS12525 Position (kb) 2264 2265Strain fitness (log2 ratio) -3 -2 -1 0 1at 2264.518 kb on + strandat 2264.540 kb on + strand, within SGL_RS12515at 2264.801 kb on + strandat 2264.903 kb on - strandat 2264.920 kb on - strandat 2265.181 kb on - strandat 2265.251 kb on + strandat 2265.311 kb on - strandat 2265.324 kb on - strandat 2265.394 kb on + strand, within SGL_RS12525at 2265.523 kb on + strand, within SGL_RS12525at 2265.548 kb on - strand, within SGL_RS12525at 2265.642 kb on + strand, within SGL_RS12525at 2265.713 kb on + strand, within SGL_RS12525

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Per-strain Table

Position Strand Gene LocusTag Fraction growth in full media supplemented with 5mM sodum metaarsenite but diluted to prevent light attenuation
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2,264,518 + -2.8
2,264,540 + SGL_RS12515 0.17 -1.1
2,264,801 + -1.6
2,264,903 - +0.2
2,264,920 - +0.1
2,265,181 - +0.1
2,265,251 + -0.4
2,265,311 - +0.3
2,265,324 - -0.1
2,265,394 + SGL_RS12525 0.13 +0.2
2,265,523 + SGL_RS12525 0.22 -0.1
2,265,548 - SGL_RS12525 0.23 -0.3
2,265,642 + SGL_RS12525 0.30 +0.3
2,265,713 + SGL_RS12525 0.35 -0.4

Or see this region's nucleotide sequence