Experiment: growth in full media supplemented with 300 .M BCSA to remove any trace of Cu but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10015 and SGL_RS10020 are separated by 26 nucleotides SGL_RS10020 and SGL_RS18875 are separated by 252 nucleotides SGL_RS18875 and SGL_RS19880 overlap by 91 nucleotides SGL_RS19880 and SGL_RS10035 are separated by 418 nucleotides
SGL_RS10015: SGL_RS10015 - calcium/proton exchanger, at 1,723,452 to 1,724,570
_RS10015
SGL_RS10020: SGL_RS10020 - dCTP deaminase, at 1,724,597 to 1,725,178
_RS10020
SGL_RS18875: SGL_RS18875 - IS630 family transposase, at 1,725,431 to 1,726,009
_RS18875
SGL_RS19880: SGL_RS19880 - IS630 transposase-related protein, at 1,725,919 to 1,726,278
_RS19880
SGL_RS10035: SGL_RS10035 - VWA domain-containing protein, at 1,726,697 to 1,727,299
_RS10035
Position (kb) 1725
1726
1727 Strain fitness (log2 ratio) -1
0
1 at 1724.481 kb on - strand at 1724.502 kb on - strand at 1724.595 kb on + strand at 1725.257 kb on + strand at 1726.899 kb on - strand, within SGL_RS10035
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 300 .M BCSA to remove any trace of Cu but diluted to prevent light attenuation remove 1,724,481 - -0.4 1,724,502 - -0.2 1,724,595 + -0.4 1,725,257 + -0.3 1,726,899 - SGL_RS10035 0.33 +0.1
Or see this region's nucleotide sequence