Experiment: growth in full media supplemented with 300 .M BCSA to remove any trace of Cu but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07905 and SGL_RS07910 are separated by 122 nucleotides SGL_RS07910 and SGL_RS07915 are separated by 74 nucleotides SGL_RS07915 and SGL_RS07920 are separated by 122 nucleotides SGL_RS07920 and SGL_RS07925 are separated by 140 nucleotides
SGL_RS07905: SGL_RS07905 - DUF29 domain-containing protein, at 1,290,496 to 1,290,957
_RS07905
SGL_RS07910: SGL_RS07910 - DUF29 domain-containing protein, at 1,291,080 to 1,291,556
_RS07910
SGL_RS07915: SGL_RS07915 - DUF29 domain-containing protein, at 1,291,631 to 1,292,092
_RS07915
SGL_RS07920: SGL_RS07920 - DUF29 domain-containing protein, at 1,292,215 to 1,292,679
_RS07920
SGL_RS07925: SGL_RS07925 - methyltransferase domain-containing protein, at 1,292,820 to 1,293,356
_RS07925
Position (kb)
1291
1292
1293 Strain fitness (log2 ratio)
-1
0
1 at 1291.442 kb on + strand, within SGL_RS07910 at 1291.472 kb on + strand, within SGL_RS07910 at 1291.577 kb on + strand at 1291.881 kb on - strand, within SGL_RS07915 at 1292.353 kb on + strand, within SGL_RS07920 at 1292.892 kb on + strand, within SGL_RS07925 at 1293.061 kb on - strand, within SGL_RS07925 at 1293.061 kb on - strand, within SGL_RS07925
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 300 .M BCSA to remove any trace of Cu but diluted to prevent light attenuation remove 1,291,442 + SGL_RS07910 0.76 -0.9 1,291,472 + SGL_RS07910 0.82 +0.2 1,291,577 + -0.7 1,291,881 - SGL_RS07915 0.54 +0.4 1,292,353 + SGL_RS07920 0.30 -0.2 1,292,892 + SGL_RS07925 0.13 +0.4 1,293,061 - SGL_RS07925 0.45 +0.2 1,293,061 - SGL_RS07925 0.45 +0.3
Or see this region's nucleotide sequence