Experiment: growth in full media supplemented with 300 .M BCSA to remove any trace of Cu but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06500 and SGL_RS06505 are separated by 6 nucleotides SGL_RS06505 and SGL_RS06510 are separated by 125 nucleotides SGL_RS06510 and SGL_RS06515 are separated by 211 nucleotides
SGL_RS06500: SGL_RS06500 - glycosyltransferase family 4 protein, at 986,492 to 987,733
_RS06500
SGL_RS06505: SGL_RS06505 - twin-arginine translocase TatA/TatE family subunit, at 987,740 to 987,946
_RS06505
SGL_RS06510: SGL_RS06510 - YdiU family protein, at 988,072 to 989,505
_RS06510
SGL_RS06515: SGL_RS06515 - ATP-dependent zinc metalloprotease FtsH4, at 989,717 to 991,603
_RS06515
Position (kb)
988
989
990 Strain fitness (log2 ratio)
-1
0
1 at 987.559 kb on + strand, within SGL_RS06500 at 987.647 kb on - strand at 988.180 kb on - strand at 988.381 kb on + strand, within SGL_RS06510 at 988.448 kb on - strand, within SGL_RS06510 at 988.470 kb on + strand, within SGL_RS06510 at 988.490 kb on - strand, within SGL_RS06510 at 988.698 kb on - strand, within SGL_RS06510 at 988.704 kb on + strand, within SGL_RS06510 at 988.881 kb on - strand, within SGL_RS06510 at 989.012 kb on - strand, within SGL_RS06510 at 989.747 kb on - strand at 989.940 kb on + strand, within SGL_RS06515 at 989.948 kb on - strand, within SGL_RS06515 at 990.122 kb on - strand, within SGL_RS06515 at 990.153 kb on - strand, within SGL_RS06515 at 990.200 kb on - strand, within SGL_RS06515 at 990.200 kb on - strand, within SGL_RS06515 at 990.272 kb on - strand, within SGL_RS06515
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 300 .M BCSA to remove any trace of Cu but diluted to prevent light attenuation remove 987,559 + SGL_RS06500 0.86 -0.4 987,647 - +0.4 988,180 - +0.0 988,381 + SGL_RS06510 0.22 +0.7 988,448 - SGL_RS06510 0.26 +0.1 988,470 + SGL_RS06510 0.28 +0.5 988,490 - SGL_RS06510 0.29 +0.2 988,698 - SGL_RS06510 0.44 +0.1 988,704 + SGL_RS06510 0.44 +0.7 988,881 - SGL_RS06510 0.56 +0.6 989,012 - SGL_RS06510 0.66 -0.3 989,747 - +0.4 989,940 + SGL_RS06515 0.12 -0.1 989,948 - SGL_RS06515 0.12 +1.5 990,122 - SGL_RS06515 0.21 +0.1 990,153 - SGL_RS06515 0.23 +0.1 990,200 - SGL_RS06515 0.26 -0.1 990,200 - SGL_RS06515 0.26 +0.2 990,272 - SGL_RS06515 0.29 -1.6
Or see this region's nucleotide sequence