Experiment: growth in full media but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10520 and SGL_RS10525 are separated by 130 nucleotides SGL_RS10525 and SGL_RS10530 are separated by 69 nucleotides SGL_RS10530 and SGL_RS10535 are separated by 71 nucleotides SGL_RS10535 and SGL_RS10540 are separated by 272 nucleotides
SGL_RS10520: SGL_RS10520 - J domain-containing protein, at 1,829,553 to 1,830,497
_RS10520
SGL_RS10525: SGL_RS10525 - inositol monophosphatase family protein, at 1,830,628 to 1,831,491
_RS10525
SGL_RS10530: SGL_RS10530 - 2Fe-2S iron-sulfur cluster-binding protein, at 1,831,561 to 1,831,929
_RS10530
SGL_RS10535: SGL_RS10535 - tRNA-Ser, at 1,832,001 to 1,832,087
_RS10535
SGL_RS10540: SGL_RS10540 - C40 family peptidase, at 1,832,360 to 1,833,169
_RS10540
Position (kb)
1830
1831
1832 Strain fitness (log2 ratio)
-1
0
1 at 1829.699 kb on - strand, within SGL_RS10520 at 1829.714 kb on - strand, within SGL_RS10520 at 1829.820 kb on + strand, within SGL_RS10520 at 1829.938 kb on + strand, within SGL_RS10520 at 1830.065 kb on - strand, within SGL_RS10520 at 1830.200 kb on + strand, within SGL_RS10520 at 1830.347 kb on + strand, within SGL_RS10520 at 1830.669 kb on + strand at 1830.675 kb on - strand at 1830.740 kb on + strand, within SGL_RS10525 at 1830.899 kb on - strand, within SGL_RS10525 at 1830.976 kb on + strand, within SGL_RS10525 at 1831.164 kb on - strand, within SGL_RS10525 at 1831.169 kb on - strand, within SGL_RS10525 at 1831.174 kb on - strand, within SGL_RS10525 at 1831.272 kb on + strand, within SGL_RS10525 at 1831.280 kb on - strand, within SGL_RS10525 at 1831.567 kb on - strand at 1831.567 kb on - strand at 1831.591 kb on + strand at 1832.103 kb on - strand at 1832.120 kb on + strand at 1832.169 kb on + strand at 1832.171 kb on + strand at 1832.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media but diluted to prevent light attenuation remove 1,829,699 - SGL_RS10520 0.15 +0.3 1,829,714 - SGL_RS10520 0.17 +0.5 1,829,820 + SGL_RS10520 0.28 +0.4 1,829,938 + SGL_RS10520 0.41 +0.3 1,830,065 - SGL_RS10520 0.54 +0.3 1,830,200 + SGL_RS10520 0.68 +0.6 1,830,347 + SGL_RS10520 0.84 -0.7 1,830,669 + -0.5 1,830,675 - +0.1 1,830,740 + SGL_RS10525 0.13 +0.3 1,830,899 - SGL_RS10525 0.31 +0.0 1,830,976 + SGL_RS10525 0.40 +0.6 1,831,164 - SGL_RS10525 0.62 -0.4 1,831,169 - SGL_RS10525 0.63 +0.3 1,831,174 - SGL_RS10525 0.63 +1.2 1,831,272 + SGL_RS10525 0.75 -0.4 1,831,280 - SGL_RS10525 0.75 +0.7 1,831,567 - +0.5 1,831,567 - +0.1 1,831,591 + -1.6 1,832,103 - +0.4 1,832,120 + -0.1 1,832,169 + +0.3 1,832,171 + -0.5 1,832,177 - +0.2
Or see this region's nucleotide sequence