Experiment: growth in full media supplemented with 3 .M Cl2Co
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14335 and SGL_RS14340 overlap by 8 nucleotides SGL_RS14340 and SGL_RS14345 are separated by 130 nucleotides SGL_RS14345 and SGL_RS14350 are separated by 14 nucleotides
SGL_RS14335: SGL_RS14335 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 2,670,115 to 2,671,902
_RS14335
SGL_RS14340: SGL_RS14340 - protein phosphatase 2C domain-containing protein, at 2,671,895 to 2,673,970
_RS14340
SGL_RS14345: SGL_RS14345 - VWA domain-containing protein, at 2,674,101 to 2,674,721
_RS14345
SGL_RS14350: SGL_RS14350 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase, at 2,674,736 to 2,676,196
_RS14350
Position (kb)
2671
2672
2673
2674 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2671.039 kb on + strand, within SGL_RS14335 at 2671.085 kb on + strand, within SGL_RS14335 at 2671.338 kb on - strand, within SGL_RS14335 at 2671.667 kb on + strand, within SGL_RS14335 at 2671.885 kb on - strand at 2671.885 kb on - strand at 2672.012 kb on - strand at 2672.077 kb on + strand at 2672.212 kb on + strand, within SGL_RS14340 at 2672.256 kb on - strand, within SGL_RS14340 at 2672.492 kb on - strand, within SGL_RS14340 at 2672.515 kb on - strand, within SGL_RS14340 at 2672.532 kb on + strand, within SGL_RS14340 at 2672.540 kb on - strand, within SGL_RS14340 at 2672.652 kb on - strand, within SGL_RS14340 at 2672.662 kb on + strand, within SGL_RS14340 at 2672.670 kb on - strand, within SGL_RS14340 at 2672.671 kb on - strand, within SGL_RS14340 at 2672.892 kb on + strand, within SGL_RS14340 at 2672.942 kb on - strand, within SGL_RS14340 at 2673.061 kb on + strand, within SGL_RS14340 at 2673.072 kb on + strand, within SGL_RS14340 at 2673.092 kb on - strand, within SGL_RS14340 at 2673.190 kb on - strand, within SGL_RS14340 at 2673.216 kb on + strand, within SGL_RS14340 at 2673.221 kb on + strand, within SGL_RS14340 at 2673.335 kb on + strand, within SGL_RS14340 at 2673.335 kb on + strand, within SGL_RS14340 at 2673.487 kb on - strand, within SGL_RS14340 at 2673.495 kb on + strand, within SGL_RS14340 at 2673.542 kb on - strand, within SGL_RS14340 at 2673.753 kb on - strand, within SGL_RS14340 at 2673.788 kb on + strand at 2673.881 kb on + strand at 2673.961 kb on - strand at 2673.967 kb on + strand at 2673.980 kb on - strand at 2673.980 kb on - strand at 2674.103 kb on - strand at 2674.215 kb on + strand, within SGL_RS14345 at 2674.334 kb on + strand, within SGL_RS14345 at 2674.368 kb on + strand, within SGL_RS14345 at 2674.368 kb on + strand, within SGL_RS14345 at 2674.376 kb on - strand, within SGL_RS14345 at 2674.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 3 .M Cl2Co remove 2,671,039 + SGL_RS14335 0.52 -2.0 2,671,085 + SGL_RS14335 0.54 -1.5 2,671,338 - SGL_RS14335 0.68 -1.5 2,671,667 + SGL_RS14335 0.87 -0.2 2,671,885 - -1.8 2,671,885 - -0.5 2,672,012 - +0.6 2,672,077 + -2.7 2,672,212 + SGL_RS14340 0.15 -1.4 2,672,256 - SGL_RS14340 0.17 -1.2 2,672,492 - SGL_RS14340 0.29 -0.9 2,672,515 - SGL_RS14340 0.30 -2.6 2,672,532 + SGL_RS14340 0.31 -1.5 2,672,540 - SGL_RS14340 0.31 -0.6 2,672,652 - SGL_RS14340 0.36 -0.8 2,672,662 + SGL_RS14340 0.37 -2.4 2,672,670 - SGL_RS14340 0.37 -0.9 2,672,671 - SGL_RS14340 0.37 -0.1 2,672,892 + SGL_RS14340 0.48 -0.8 2,672,942 - SGL_RS14340 0.50 -0.4 2,673,061 + SGL_RS14340 0.56 -3.3 2,673,072 + SGL_RS14340 0.57 -2.5 2,673,092 - SGL_RS14340 0.58 -1.2 2,673,190 - SGL_RS14340 0.62 -2.8 2,673,216 + SGL_RS14340 0.64 -2.3 2,673,221 + SGL_RS14340 0.64 -1.6 2,673,335 + SGL_RS14340 0.69 -1.6 2,673,335 + SGL_RS14340 0.69 -2.4 2,673,487 - SGL_RS14340 0.77 -2.5 2,673,495 + SGL_RS14340 0.77 -2.5 2,673,542 - SGL_RS14340 0.79 -0.1 2,673,753 - SGL_RS14340 0.89 -2.2 2,673,788 + -1.5 2,673,881 + +0.8 2,673,961 - -2.2 2,673,967 + -1.8 2,673,980 - -3.2 2,673,980 - -2.4 2,674,103 - -0.1 2,674,215 + SGL_RS14345 0.18 +1.3 2,674,334 + SGL_RS14345 0.38 -0.2 2,674,368 + SGL_RS14345 0.43 +0.3 2,674,368 + SGL_RS14345 0.43 +0.0 2,674,376 - SGL_RS14345 0.44 +0.4 2,674,724 - +0.0
Or see this region's nucleotide sequence