Experiment: growth in full media supplemented with 5mM sodum metaarsenite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03055 and SGL_RS03060 are separated by 146 nucleotides SGL_RS03060 and SGL_RS03065 are separated by 51 nucleotides SGL_RS03065 and SGL_RS03070 are separated by 196 nucleotides SGL_RS03070 and SGL_RS03075 are separated by 57 nucleotides
SGL_RS03055: SGL_RS03055 - protoporphyrinogen oxidase HemJ, at 256,647 to 257,279
_RS03055
SGL_RS03060: SGL_RS03060 - phosphoadenosine phosphosulfate reductase, at 257,426 to 258,175
_RS03060
SGL_RS03065: SGL_RS03065 - heat-inducible transcriptional repressor HrcA, at 258,227 to 259,363
_RS03065
SGL_RS03070: SGL_RS03070 - tRNA-Ser, at 259,560 to 259,644
_RS03070
SGL_RS03075: SGL_RS03075 - shikimate kinase, at 259,702 to 260,271
_RS03075
Position (kb)
258
259
260 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 257.240 kb on + strand at 257.359 kb on - strand at 257.397 kb on - strand at 257.397 kb on - strand at 257.428 kb on + strand at 257.691 kb on + strand, within SGL_RS03060 at 257.699 kb on - strand, within SGL_RS03060 at 257.711 kb on + strand, within SGL_RS03060 at 257.866 kb on - strand, within SGL_RS03060 at 258.024 kb on - strand, within SGL_RS03060 at 258.052 kb on + strand, within SGL_RS03060 at 258.056 kb on + strand, within SGL_RS03060 at 258.297 kb on - strand at 258.335 kb on - strand at 258.619 kb on - strand, within SGL_RS03065 at 258.853 kb on + strand, within SGL_RS03065 at 259.099 kb on - strand, within SGL_RS03065 at 259.149 kb on + strand, within SGL_RS03065 at 259.177 kb on - strand, within SGL_RS03065 at 259.262 kb on - strand at 259.264 kb on + strand at 259.264 kb on + strand at 259.272 kb on - strand at 259.272 kb on - strand at 259.498 kb on - strand at 259.692 kb on - strand at 259.727 kb on + strand at 259.727 kb on + strand at 259.728 kb on - strand at 260.279 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5mM sodum metaarsenite remove 257,240 + -2.1 257,359 - +0.4 257,397 - -0.2 257,397 - +0.1 257,428 + -0.2 257,691 + SGL_RS03060 0.35 +0.1 257,699 - SGL_RS03060 0.36 +0.6 257,711 + SGL_RS03060 0.38 +0.6 257,866 - SGL_RS03060 0.59 -0.3 258,024 - SGL_RS03060 0.80 +0.0 258,052 + SGL_RS03060 0.83 -0.0 258,056 + SGL_RS03060 0.84 +0.3 258,297 - -2.2 258,335 - -1.1 258,619 - SGL_RS03065 0.34 -1.5 258,853 + SGL_RS03065 0.55 -1.1 259,099 - SGL_RS03065 0.77 -0.9 259,149 + SGL_RS03065 0.81 -0.4 259,177 - SGL_RS03065 0.84 -1.0 259,262 - -2.3 259,264 + -1.0 259,264 + +0.7 259,272 - -1.1 259,272 - +0.5 259,498 - +0.1 259,692 - +0.5 259,727 + +2.2 259,727 + +0.3 259,728 - +1.6 260,279 + -0.2
Or see this region's nucleotide sequence