Experiment: growth in full media supplemented with 3 .M CuSO4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12860 and SGL_RS12865 are separated by 37 nucleotides SGL_RS12865 and SGL_RS12870 are separated by 26 nucleotides SGL_RS12870 and SGL_RS12875 are separated by 372 nucleotides
SGL_RS12860: SGL_RS12860 - NCS2 family permease, at 2,344,152 to 2,345,759
_RS12860
SGL_RS12865: SGL_RS12865 - pseudouridine synthase, at 2,345,797 to 2,346,546
_RS12865
SGL_RS12870: SGL_RS12870 - YqeG family HAD IIIA-type phosphatase, at 2,346,573 to 2,347,127
_RS12870
SGL_RS12875: SGL_RS12875 - DUF3769 domain-containing protein, at 2,347,500 to 2,349,911
_RS12875
Position (kb)
2346
2347
2348 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2345.575 kb on + strand, within SGL_RS12860 at 2345.666 kb on - strand at 2345.671 kb on - strand at 2345.748 kb on + strand at 2345.795 kb on - strand at 2346.100 kb on - strand, within SGL_RS12865 at 2346.217 kb on - strand, within SGL_RS12865 at 2346.236 kb on - strand, within SGL_RS12865 at 2346.297 kb on + strand, within SGL_RS12865 at 2346.434 kb on + strand, within SGL_RS12865 at 2346.531 kb on + strand at 2346.670 kb on - strand, within SGL_RS12870 at 2346.689 kb on + strand, within SGL_RS12870 at 2346.690 kb on + strand, within SGL_RS12870 at 2346.691 kb on - strand, within SGL_RS12870 at 2346.888 kb on - strand, within SGL_RS12870 at 2347.093 kb on + strand at 2347.101 kb on - strand at 2347.104 kb on + strand at 2347.488 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 3 .M CuSO4 remove 2,345,575 + SGL_RS12860 0.88 +0.0 2,345,666 - +0.4 2,345,671 - -0.6 2,345,748 + -0.4 2,345,795 - -0.6 2,346,100 - SGL_RS12865 0.40 +0.1 2,346,217 - SGL_RS12865 0.56 +0.4 2,346,236 - SGL_RS12865 0.59 -0.2 2,346,297 + SGL_RS12865 0.67 -0.1 2,346,434 + SGL_RS12865 0.85 +0.3 2,346,531 + +1.3 2,346,670 - SGL_RS12870 0.17 -3.5 2,346,689 + SGL_RS12870 0.21 -3.0 2,346,690 + SGL_RS12870 0.21 -3.4 2,346,691 - SGL_RS12870 0.21 -1.4 2,346,888 - SGL_RS12870 0.57 -1.9 2,347,093 + -0.7 2,347,101 - -4.2 2,347,104 + -2.3 2,347,488 - -0.3
Or see this region's nucleotide sequence