Experiment: growth in full media supplemented with 3 .M CuSO4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05255 and SGL_RS05260 are separated by 170 nucleotides SGL_RS05260 and SGL_RS05265 are separated by 186 nucleotides SGL_RS05265 and SGL_RS05270 are separated by 236 nucleotides
SGL_RS05255: SGL_RS05255 - hypothetical protein, at 728,271 to 728,489
_RS05255
SGL_RS05260: SGL_RS05260 - hypothetical protein, at 728,660 to 729,484
_RS05260
SGL_RS05265: SGL_RS05265 - hypothetical protein, at 729,671 to 729,871
_RS05265
SGL_RS05270: SGL_RS05270 - serine/threonine-protein kinase, at 730,108 to 731,674
_RS05270
Position (kb) 728
729
730 Strain fitness (log2 ratio) -2
-1
0
1 at 727.847 kb on + strand at 728.248 kb on - strand at 728.531 kb on - strand at 728.847 kb on - strand, within SGL_RS05260 at 728.874 kb on - strand, within SGL_RS05260 at 728.990 kb on - strand, within SGL_RS05260 at 729.197 kb on - strand, within SGL_RS05260 at 729.291 kb on - strand, within SGL_RS05260 at 729.425 kb on + strand at 729.425 kb on + strand at 729.432 kb on + strand at 729.780 kb on - strand, within SGL_RS05265 at 729.800 kb on - strand, within SGL_RS05265 at 729.952 kb on + strand at 730.072 kb on + strand at 730.072 kb on + strand at 730.072 kb on + strand at 730.080 kb on - strand at 730.148 kb on - strand at 730.221 kb on + strand at 730.375 kb on - strand, within SGL_RS05270 at 730.402 kb on - strand, within SGL_RS05270
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 3 .M CuSO4 remove 727,847 + +0.0 728,248 - -0.4 728,531 - -0.7 728,847 - SGL_RS05260 0.23 -2.1 728,874 - SGL_RS05260 0.26 -1.4 728,990 - SGL_RS05260 0.40 -1.2 729,197 - SGL_RS05260 0.65 -1.7 729,291 - SGL_RS05260 0.76 -2.5 729,425 + -1.0 729,425 + -0.4 729,432 + -1.1 729,780 - SGL_RS05265 0.54 +0.9 729,800 - SGL_RS05265 0.64 -0.3 729,952 + +0.1 730,072 + +0.3 730,072 + -0.1 730,072 + +0.0 730,080 - -0.1 730,148 - -0.1 730,221 + +0.3 730,375 - SGL_RS05270 0.17 +0.5 730,402 - SGL_RS05270 0.19 -0.4
Or see this region's nucleotide sequence