Experiment: Arg 10 mM as N source, buffered with TES 20 mM TES pH8 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16765 and SGL_RS16770 are separated by 144 nucleotides SGL_RS16770 and SGL_RS16775 are separated by 43 nucleotides
SGL_RS16765: SGL_RS16765 - aldo/keto reductase, at 3,220,162 to 3,221,106
_RS16765
SGL_RS16770: SGL_RS16770 - LptF/LptG family permease, at 3,221,251 to 3,222,432
_RS16770
SGL_RS16775: SGL_RS16775 - asparagine--tRNA ligase, at 3,222,476 to 3,223,864
_RS16775
Position (kb)
3221
3222
3223 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3220.263 kb on - strand, within SGL_RS16765 at 3220.308 kb on - strand, within SGL_RS16765 at 3220.448 kb on + strand, within SGL_RS16765 at 3220.458 kb on + strand, within SGL_RS16765 at 3220.464 kb on + strand, within SGL_RS16765 at 3220.663 kb on + strand, within SGL_RS16765 at 3221.355 kb on + strand at 3221.384 kb on - strand, within SGL_RS16770 at 3221.521 kb on - strand, within SGL_RS16770 at 3221.583 kb on - strand, within SGL_RS16770 at 3222.146 kb on - strand, within SGL_RS16770 at 3222.184 kb on - strand, within SGL_RS16770 at 3222.204 kb on - strand, within SGL_RS16770 at 3222.348 kb on + strand at 3222.539 kb on - strand at 3222.540 kb on + strand at 3222.545 kb on + strand at 3222.610 kb on + strand at 3222.618 kb on - strand, within SGL_RS16775 at 3222.618 kb on - strand, within SGL_RS16775 at 3223.148 kb on + strand, within SGL_RS16775 at 3223.211 kb on + strand, within SGL_RS16775 at 3223.211 kb on + strand, within SGL_RS16775 at 3223.241 kb on + strand, within SGL_RS16775 at 3223.249 kb on + strand, within SGL_RS16775 at 3223.257 kb on - strand, within SGL_RS16775 at 3223.257 kb on - strand, within SGL_RS16775 at 3223.351 kb on - strand, within SGL_RS16775 at 3223.396 kb on - strand, within SGL_RS16775
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with TES 20 mM TES pH8 but diluted to prevent light attenuation remove 3,220,263 - SGL_RS16765 0.11 +0.4 3,220,308 - SGL_RS16765 0.15 -0.0 3,220,448 + SGL_RS16765 0.30 +0.0 3,220,458 + SGL_RS16765 0.31 -0.2 3,220,464 + SGL_RS16765 0.32 -0.2 3,220,663 + SGL_RS16765 0.53 -0.2 3,221,355 + -1.5 3,221,384 - SGL_RS16770 0.11 -3.9 3,221,521 - SGL_RS16770 0.23 -4.0 3,221,583 - SGL_RS16770 0.28 -4.1 3,222,146 - SGL_RS16770 0.76 +0.3 3,222,184 - SGL_RS16770 0.79 -2.8 3,222,204 - SGL_RS16770 0.81 -1.1 3,222,348 + -1.6 3,222,539 - -0.0 3,222,540 + -0.4 3,222,545 + -1.6 3,222,610 + -0.2 3,222,618 - SGL_RS16775 0.10 +0.4 3,222,618 - SGL_RS16775 0.10 -0.4 3,223,148 + SGL_RS16775 0.48 +0.3 3,223,211 + SGL_RS16775 0.53 -0.1 3,223,211 + SGL_RS16775 0.53 -0.2 3,223,241 + SGL_RS16775 0.55 -0.0 3,223,249 + SGL_RS16775 0.56 -0.7 3,223,257 - SGL_RS16775 0.56 +0.9 3,223,257 - SGL_RS16775 0.56 -0.4 3,223,351 - SGL_RS16775 0.63 +0.9 3,223,396 - SGL_RS16775 0.66 +0.3
Or see this region's nucleotide sequence